نتایج جستجو برای: standard pcr

تعداد نتایج: 683958  

Journal: :Medical research archives 2021

Although blood culture is considered the gold standard for detection of neonatal infections bloodstream, its result can take from 48-72 h and has low sensitivity. In this study, we determined usefulness panbacterial polymerase chain reaction (PCR) panviral RT-PCR in serum, as tests early diagnosis sepsis. We studied 195 patients with clinical sepsis, up to age 28 days, hospitalized Neonatal Int...

Journal: :acta medica iranica 0
reza shahsiah department of pathology, imam hospitals complex, school of medicine, tehran university of medical sciences, tehran, iran. alireza abdollahi department of pathology, imam hospitals complex, school of medicine, tehran university of medical sciences, tehran, iran. farid azmoudeh ardalan department of pathology, imam hospitals complex, school of medicine, tehran university of medical sciences, tehran, iran. mohammad taghi haghi-ashtiani pediatrics center of excellence, children's medical center, tehran university of medical sciences, tehran, iran. issa jahanzad department of pathology, imam hospitals complex, school of medicine, tehran university of medical sciences, tehran, iran. mohsen nassiri toosi department of gastroenterology, school of medicine, tehran university of medical sciences, tehran, iran.

fluorescent monitoring of dna amplification is the basis of real-time pcr. absolute quantification can be achieved using a standard curve method. the standard curve is constructed by amplifying known amounts of standards under identical conditions to that of the samples.the objective of the current study is to propose a mathematical model to assess the acceptability of pcr resulys.four commerci...

Journal: :Turkish journal of haematology : official journal of Turkish Society of Haematology 2008
Duran Ustek Sema Sırma Aris Cakiris Fulya Coşan Başar Oku Uğur Özbek

The diagnosis of leukemia-specific mRNAs by polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR) require well-known positive standard controls. In general, the positive controls are obtained from cell lines and leukemia patients who have been diagnosed at the molecular level by RT-PCR. These are expensive and restricted sources for standard positive controls. Thus, there is a ...

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