Response surface methodology was employed to optimize the composition of medium for the production of avermectin B1a by Streptomyces avermitilis 14-12A in shaker flask cultivation. Corn starch and yeast extract were found to have significant effects on avermectin B1a production by the Plackett-Burman design. The steepest ascent method was used to access the optimal region of the medium composit...

Streptomyces sp. JAJ06 is a seawater-dependent antibiotic producer, previously isolated and characterised from an Indian coastal solar saltern. This paper reports replacement of seawater with a defined salt formulation in production medium and subsequent statistical media optimization to ensure consistent as well as improved antibiotic production by Streptomyces sp. JAJ06. This strain was obser...

The ability of Methicillin-resistant Staphylococcus aureus (MRSA) to form biofilms is one the triggering factors for emergence MRSA resistance antibiotics. Streptomyces W-5B has shown potency as an antibacterial producer against MRSA. However, production microbial bioactive compounds strongly affected by source nutrients in fermentation medium. Therefore, objective this study was determine opti...

Optimization of alkaline protease production by Streptomyces ambofaciens NRRL 2420 in free and immobilized form was investigated using submerged fermentation technique. The optimum conditions for maximum alkaline protease production 342 unit mL were 30°C at pH 8.5 and incubation time 96 h in free cell cultures using starch 20 g L as carbon source and yeast extract 5 g L as nitrogen source. The ...

Marine actinobacteria are known to be a rich source for novel metabolites with diverse biological activities. In this study, a potential extracellular L-asparaginase was characterised from the Streptomyces griseus NIOT-VKMA29. Box-Behnken based optimization was used to determine the culture medium components to enhance the L-asparaginase production. pH, starch, yeast extract and L-asparagine ha...

A Streptomyces Ds-104 isolate was found to inhibit four multi-drug resistant Candida albicans pathogens with an optimal production of the inhibitory compound(s) in starch, casein, nitrate broth (SCNB) and inhibition zone diameters ranging between 14 and 18 mm. These were significantly different (P= 0.05) from what was obtained in nutrient, oatmeal, inorganic salt starch, and yeast extract, malt...

A survey was carried out to select an antibiotic producing Streptomyces strain isolated from garden soil in Alexandria, Egypt. Streptomyces violatus showed the highest antimicrobial activity in static cultures after 7 days incubation at 30°C. The antibacterial substance was more active against Bacillus subtilis and Staphyllococcus aureus than Escherichia coli or Sarcina lutea. Growth of S. viol...

The biological activity of Streptomyces aureofaciens MY18 and Streptomyces roseviolaceus MR13 (local isolates) as influenced by temperature,aeration, different media and carbon and nitrogen sources were investigated. It was found that biomass., growth parameters and antibiotic activity of bOth strains were highly affected by these factors. The first strain showed higher temperature range than t...