Background: In this study, we examined a new two step induction protocol for improving the differentiation of human umbilical cord blood-derived mesenchymal stem cells into neural progenitor cells. Materials and Methods: Human umbilical cord blood-derived mesenchymal stem cells were first cultured in Dulbecco’s modified eagle medium supplemented with 10% fetal bovine serum in a humidified incu...

conclusions taken together, the combination of chemical and growth factors in the two-step induction protocol may improve the efficiency of msc differentiation into neural progenitor cells, and may be a new method for the easy and fast application of mscs in regenerative medicine in the future. results the results of real-time pcr showed that the expression of the gfap, mbp, and map-2 genes aft...

backgrounds: a straight-forward way to identify whether a drug or environmental chemical can be harmful to unborn baby is to evaluate its effect on laboratory animals. all invivo methods need large number of animal and are therefore time consuming and expensive. however, the thousands of chemicals in need of testing, to reduce the spending of live animals, an assortment of in vitro assays has b...

background: the anticancer agent imatinib (im) is a small molecular analog ofatp that inhibits tyrosine kinase activity of platelet derived growth factors (pdgfs)and stem cell factor (scf) receptor in cancer cells. however these factors have a keyrole in regulating growth and development of normal sertoli, leydig and germcells.objective: the aim of this study was to determine cell viability, pd...

Background and Aims: Bone marrow-derived mesenchymal stem cells (BM-MSCs) are a well-known source of multipotent adult stem cells. Despite using different methodologies of MSCs preparing for clinical applications, the top safest procedure to manipulate these cells, has not yet been determined. Recently, ex-vivo expansion of MSCs for their subsequent implantation, using some biological product, ...

background: retinoic acid as one of the most important regulators for cell differentiation was examined in this study for differentiation of human umbilical mesenchymal cells (hucm). methods: after isolation, hucm were evaluated for mesenchymal stem cell properties by flow cytometry and alkaline phosphatase assay. also, doubling time of the cells and their differentiation potential into adipoge...

introduction: embryonic stem (es) cells are pluripotent cells that derived from inner cell mass (icm) of blastocyst stage and are able to differentiate in vitro into different cells. purpose: this study was initiated to evaluate the effect of basic fibroblast growth factor (bfgf) on differentiation of es cells into cardiomyocytes among with their response to cardiac drugs. methods: the mouse em...

research about potential use of stem cells for the development of germ line cells in vitro had been challenged. in the present study, we reported a novel protocol consisting of cocktail growth factor addition for germ cell differentiation followed by transfection. the cells were purificated based on the expression on the cell surface of a protein. this protein is not present in normal cells of ...

Transforming growth factor-beta (TGF-beta) and TGF-beta-related proteins, such as the bone morphogenetic proteins, have emerged as key regulators of stem cell renewal and differentiation. These proteins have disparate roles in regulating the biology of embryonic stem cells and tumor suppression, and they help define the selection of cell fate and the progression of differentiation along a linea...