OBJECTIVE (1) To assess the personality profiles of the anabolic androgenic steroid users (AAS) and (2) to determine whether valid premorbid personality traits could be obtained from cross sectional assessment using multisource data. METHODS The first author became a participant-observer in a group of body builders. An experimental group of body builders who had been using AAS for no more tha...

A screening of structurally different steroid hormone synthesis inhibitors was performed in order to find a starting point for the development of a new inhibitor of the bifunctional steroidogenic enzyme CYP17A1. Emphasis was placed on determination of selectivity between the two catalytic steps, namely 17α-hydroxylase and C(17,20)-lyase. For that purpose a new inhibition assay has been develope...

The present study describes the biological in vitro and in vivo evaluation of 2-methoxy derivatives of estrogenic inhibitors of steroid sulfatase, namely 3-sulfamoyloxy-17 -p-tert-butylbenzyl(or benzyl)-1,3,5 (10)estratrien-17 -ols. The addition of the 2-methoxy group conserves the potent inhibitory effect on steroid sulfatase activity (IC50s of 0.024 and 0.040 nM) while removing the estrogenic...

3alpha,7alpha-Dihydroxy-5-epiaragusterol A (3) was synthesized from bile acid (cholic acid) as a new steroidal nuclear analogue of antitumor marine steroid aragusterol A. 7alpha-Hydroxyaragusterol A (4) was also derived from xestokerol B. The in vitro anti-proliferative activity of each of these analogues toward KB cells as well as in vivo anti-tumor activity of 5-epiaragusterol A (2) previousl...

Prolyl hydroxylase and other enzymes of collagen synthesis have been found in cloned cells, including epithelium, melanoma, liver, HeLa, pituitary, kidney, and neuroblastoma. The significance of collagen biosynthesis in nonfibroblastic cells lines is discussed.

1. A method in use for the extraction of urinary steroid conjugates has been applied to study the recovery of synthetic steroid monoglucuronides from aqueous solution. 2. In the presence of dissolved ammonium sulphate (50g./100ml.), ether-ethanol (3:1, v/v, 3x0.5vol.) extracted the monoglucuronides of steroids of the C(18), C(19) and C(21) series, quantitatively at values pH2-9. 3. The hydrolys...

A method for gas-liquid chromatographic separation and quantitation of urinary steroid metabolites is described. A normal reference range has been established and classic examples of urinary steroid profiles of the major steroid disorders are given.

The urinary output of hypophyseal gonadotropin by normal women has proven exceedingly variable from month to month (1, 2). Thus, the usefulness of gonadotropin assays for clinical purposes has been disappointing and is now limited to the demonstration of large excesses of hormone. The status of the gonadotropin assay as a potential diagnostic measure in men is still in doubt. More normal data a...

The urinary excretion of androsterone, aetiocholanolone, total 17-oxosteroids, and 17-hydroxycorticosteroids (17-OHCS) was measured in 40 patients with lung cancer three days before resection and again 10-15 days after resection of their lung tumours. There was a significant postoperative increase in the excretion of 17-OHCS but a significant decrease in the excretion of androsterone and aetioc...

A novel homozygous long-range deletion of the CYP17A1 gene abolished protein expression and caused the severest form of 17-hydroxylase deficiency in one kindred of a Turkish family. The affected subjects presented with 46,XY sex reversal and 46,XX lack of pubertal development as well as severe hypertension.