objective: resin cements, regardless of their biocompatibility, have been widely used in restorative dentistry during the recent years. these cements contain hydroxy ethyl methacrylate (hema) molecules which are claimed to penetrate into dentinal tubules and may affect dental pulp. since tooth preparation for metal ceramic restorations involves a large surface of the tooth, cytotoxicity of thes...

the effect of natural killer (nk) cells and lymphokine activated killer ( lak) cells was studied on a group of human melanoma cell lines. peripheral blood from healthy volunteers was utilized as a fresh source of natural killer cells and rhi l-2 for producing lak cells. the cytotoxicity of effector cells was quantified using a 4 hour si determining the density of antigen expression on tumor cel...

Quantum dot (QD) nanoparticles have potential applications in nanomedicine as drug delivery vectors and diagnostic agents, but the skin toxicity and irritation potential of QDs are unknown. Human epidermal keratinocytes (HEKs) were used to assess if QDs with different surface coatings would cause differential effects on HEK cytotoxicity, proinflammatory cytokine release, and cellular uptake. Co...

Clostridium perfringens iota-toxin is a binary toxin consisting of iota a (Ia), an ADP-ribosyltransferase that modifies actin, and iota b (Ib), which binds to a cell surface protein and translocates Ia into a target cell. Fusion proteins of recombinant Ib and truncated variants were tested for binding to Vero cells and docking with Ia via fluorescence-activated cytometry and cytotoxicity experi...

Senescent cell accumulation in aging tissues is linked to age-associated diseases and declining function, prompting efforts to eliminate them. Mass spectrometry analysis revealed that DPP4 (dipeptidyl peptidase 4) was selectively expressed on the surface of senescent, but not proliferating, human diploid fibroblasts. Importantly, the differential presence of DPP4 allowed flow cytometry-mediated...

Systemic toxicity and poor solubility of existing chemotherapeutic drugs piqued an interest in the use of nanocrystals for chemotherapy. To increase cytotoxicity, surface coating of nanocrystals is of interest to enhance tumor targeting and reduce treatment toxicity. As such, we tested in this project various coated paclitaxel nanocrystals on cancer cells for determining the efficacy of surface...

Cytotoxicity is a severe problem for cadmium sulfide nanoparticles (CSNPs) in biological systems. In this study, mercaptoacetic acid-coated CSNPs, typical semiconductor Q-dots, were synthesized in aqueous medium by the arrested precipitation method. Then, amino-terminated polyethylene glycol (PEG) was conjugated to the surface of CSNPs (PCSNPs) in order to introduce amino groups to the surface....

The role of the surface properties nanoparticles in serum protein adsorption, cellular uptake and cytotoxicity is reported. regulate their ability to adsorb proteins determine biological outcomes.