The minor groove binding asymmetric cyanine dye 4-[(3-methyl-6-(benzothiazol-2-yl)-2,3-dihydro- (benzo-1,3-thiazole)-2-methylidene)]-1-methyl-pyridin ium iodide (BEBO) is tested as sequence non- specific label in real-time PCR. The fluorescence intensity of BEBO increases upon binding to double-stranded DNA allowing emission to be measured at the end of the elongation phase in the PCR cycle. BE...

The SYBR green I (SG) dye-based fluorescence assay for screening antimalarial compounds is based on direct quantitation of parasite DNA. We show that DNA-interacting cationic cell-penetrating peptides (CPPs) and intercalating agents compete with SG dye to bind to DNA. Therefore, readouts of this assay, unlike those of the [(3)H]hypoxanthine incorporation assay, for the antimalarial activity of ...

Molecular Probes’ new generation of fluorescent nucleic acid gel stains — the SYBR Gold, SYBR Green I and SYBR Green II dyes — are by far the best high-sensitivity reagents for staining DNA (Figure 8.60) and RNA (Figure 8.61) in electrophoretic gels.1 These gel stains provide greater sensitivity with lower background fluorescence than the conventional gel stain, ethidium bromide. In addition, A...

Cat # Product Name Unit Size S-7563 SYBR Green I nucleic acid gel stain *10,000X concentrate in DMSO* ............................................................................................................... 500 μL S-7567 SYBR Green I nucleic acid gel stain *10,000X concentrate in DMSO* ..........................................................................................................

The human DNA quantification (H-Quant) system, developed for use in human identification, enables quantitation of human genomic DNA in biological samples. The assay is based on real-time amplification of AluYb8 insertions in hominoid primates. The relatively high copy number of subfamily-specific Alu repeats in the human genome enables quantification of very small amounts of human DNA. The olig...

Real-time PCR technology may provide an accurate and sensitive method to quantify hepatitis C virus (HCV) RNA. So far, studies have been carried out using the Taqman technology with the ABI Prism 7700 sequence detector. An alternative and simple real-time PCR assay is described with no probe requirement, based on the SYBR Green I dye and LightCycler fluorimeter. Amplicon synthesis was monitored...

background: nowadays, scourge of malaria as a fatalistic disease has increased due to emergence of drug resistance and tolerance among different strains of plasmodium falciparum . emergence of chloroquine (cq) resistance has worsened the calamity as cq is still considered the most efficient, safe and cost effective drug among other antimalarials. this urged the scientists to search for other al...

Real-time RT-PCR and SYBR green I melt curve analysis of a 74 bp amplicon enabled identification of Plum pox virus strains C, EA, and W, with distinct T(m)'s associated with each strain. This test is a useful supplement to a real-time RT-PCR test described earlier that was used to distinguish PPV strains D and M. A longer fragment of 155 bp was not effective for strain identification. A simplif...

background: asthma is caused by the combination of different factors. current concepts of asthma pathogenesis emphasize on gene-environment interactions. mega-genome scanning projects revealed that different single nucleotide polymorphisms (snps) are related to asthma susceptibility. rs7216389-t is one of them that is related to childhood asthma and its effect on childhood asthma severity has b...

Doxycycline, a new generation tetracycline antibiotic, is currently one of choice for the treatment and prevention of infections caused by agents of biowarfare. We are developing real time PCR assays to detect tetracycline resistance genes in Gram-negative bacteria. The assay was developed as a multiplex SYBR Green I detection using the Roche Lightcycler and multi-melting peak analysis followed...