OBJECTIVES In thrombocytopenia, high accuracy and precision of low platelet count is essential for appropriate decisions. The recently introduced Sysmex XN2000 analyzer (Sysmex, Kobe, Japan) offers 3 methods for platelet counting: impedance (PLT-I), optical (PLT-O), and a new fluorescence method (PLT-F). The precision of the PLT-F method in blood samples with platelet counts less than 50 × 10(3...

We established age- and gender-specific reference ranges for the 36 routine complete blood cell (CBC) and 57 cell population data (CPD) items in the Sysmex XN-2000 (Sysmex, Japan). In total, 280 peripheral blood samples were obtained from an equal number of healthy adults. Values for 36 routine items and 57 CPD items were obtained for each sample, and the results were categorized into six subgr...

An AutoAnalyzer method for serum iron was found to be giving erroneously high values owing to a Donnan equilibrium effect on the rate of dialysis of ferrous ions. The addition of neutral salt to the reagent corrected the deficiency. The validity of other assays on the AutoAnalyzer that measure dialyzable ions in the presence of relatively high concentrations of protein should be verified in vie...

We assessed the usefulness of gas-liquid chromatography forthe routine and reference measurement of cholesterol and triglycerides (triacylglycerols). Compared with results obtained by an AutoAnalyzer II method, correlation was good with both serum specimens (triglycerides, r = 0.88; cholesterol, r = 0.90) and lipoprotein fractions isolated by ultracentrifugation (triglycerides, r = 0.98; choles...

Nucleated red blood cells (NRBC) in blood samples interfere with the white blood cell (WBC) count on many types of automated haematology analysers. This makes it necessary to correct the WBC count by counting NRBC microscopically. This report describes the evaluation of two analysers, the Cell-Dyn 4000 and the Sysmex XE-2100, which use new techniques to recognize and enumerate NRBC. We conclude...

The Precision Quantum-in-dium (Q.I.D.) Glucometer was used to determine the glucose concentrations of 38 human blood samples. The same samples were also run on the OPERA Chemistry Autoanalyzer 2010 system. More than 44% of the glucometer reports had a difference of greater than 15% from the respective autoanalyzer reports. The calibration of the glucometer could be the source of the error and a...

In this semiautomated method, an AutoAnalyzer II is used to measure the enzymic production of glyceraldehyde 3-phosphate in hemolysates, to assay erythrocyte transketolase (EC 2.2.1.1) activity. Hemolysate and indicator reactions are separated by dialysis to eliminate hemoglobin interference and increase sensitivity. Internal standards of glyceraldehyde 3-phosphate in hemolysate carriers were q...

A method comparison study for estimation of serum Calcium and Phosphorus levels and their alterations in CKD (Chronic Kidney Disease) patients was done. Serum Calcium was estimated by O-cresolphthalein complexone spectrophotometric method and NM BAPTA autoanalyzer method. Serum Phosphorus was estimated by the Gomorri’s spectrophotometric method and Phosphomolybdate UV autoanalyzer method. The n...

In this department, haemoglobinometry has been routinely carried out for more than six years on an AutoAnalyzer. The original oxyhaemoglobin method was described in this journal (Nelson and Lamont, 1961). The possibility of adapting this analytical technique to the estimation of haemoglobin by the more stable cyanmethaemoglobin method was tested by us and reported to be satisfactory at the Symp...