dna amplification using taq dna polymerase is one of the most widely used techniques in molecular biology and biotechnology. the aim of this study was to amplify the gene of this enzyme from a thermophilic bacteria called thermus aqauticus and clone it into a vector for future use. using specific primers the cdna of taq dna polymerase was amplified and ligated into the cloning vector ptz57r usi...

By using the matrix exponential operator, solution of the system can be written as y(t) = exp(−tA)v. Numerical algorithms, which are based on this approach, are called exponential time integration methods. The essential point is that not the matrix exponential itself but rather its action on the vector v is computed. An attractive feature of the formula y(t) = exp(−tA)v is that it provides solu...

which can also be thought of as the number of paths from the lower-left corner to the upper-right corner of a k by n− k box, using unit steps up or right. insert Young diagram. The tangent space at pA is Hom(S,Q), where S is the vector space corresponding to pA, and Q = C /S. This has basis {ea ⊗ eb | a ∈ A, b 6∈ A} so the characters of the torus action are ta − tb, for a ∈ A and b 6∈ A. Given ...

Micro-dystrophins are highly promising candidates for treating Duchenne muscular dystrophy, a lethal muscle disease caused by dystrophin deficiency. Here, we report robust disease rescue in the severe DBA/2J-mdx model with a neuronal nitric oxide synthase (nNOS)-binding micro-dystrophin vector. 2 × 1013 vector genome particles/mouse of the vector were delivered intravenously to 10-week-old mice...

During the last few decades, the recombinant protein expression finds more and more applications. The cloning of protein-coding genes into expression vectors is required to be directional for proper expression, and versatile in order to facilitate gene insertion in multiple different vectors for expression tests. In this study, the TA-GC cloning method is proposed, as a new, simple and efficien...

INTRODUCTION To construct a eukaryotic expression vector of the tumour suppressor in lung cancer 1 (TSLC1) gene, so as to explore the mechanisms of tumour suppression of the gene theoretically. MATERIAL AND METHODS The open reading frame (ORF) of TSLC1 gene was amplified with RT-PCR from normal human foreskin acrobystia, and cloned to pMD19-T simple vector (TA Clone method). The resultant pla...

In this paper, the innovative intelligent fuzzy weighted input estimation method (FWIEM) can be applied to the inverse heat transfer conduction problem (IHCP) to estimate the unknown time-varying heat flux efficiently as presented. The feasibility of this method can be verified by adopting the temperature measurement experiment. We would like to focus attention on the heat flux estimation to th...

The development of a T7 RNA polymerase (T7 RNAP) expressing cell line i.e. BSR T7/5 cells marks an improvement reverse genetics for the recovery recombinant Newcastle disease virus (rNDV). is developed by transient transfection plasmid encoding RNAP gene rNDV rescue. However, expression decreases gradually over multiple passages and eventually hinders rescue rNDV. To address this issue, lentivi...

Let TA denote the space underlying the tensor algebra of a vector space A. In this short note, we show that if A is a differential graded algebra, then TA is a differential BatalinVilkovisky algebra. Moreover, if A is an A∞ algebra, then TA is a commutative BV∞ algebra. 1. Main Statement Let (A, dA) be a complex over a commutative ring R. Our convention is that dA is of degree +1. The space TA ...