Extracellular tannase production by Trichophyton rubrum has been evaluated using submerged fermentation (SmF) at different abiotic factors like pH, temperatures and biotic factors like carbon, nitrogen and concentration of gallic acid. T. rubrum produced the highest tannase level at a temperature of 30°C where the enzyme production was 23.52 U/mL and pH of 5.5 where enzyme production was 32.62 ...

Tannin acyl hydrolase (E.C.3.1.1.20) is commonly referred as tannase, hydrolyses ester and depside bonds of hydrolysable tannins to produce gallic acid, glucose and galloyl esters. Tannase has many industrial applications. The enzyme has potential uses in the treatment of tannery effluents. A lot of research is done on production aspects of tannase, but is still considered as one of the costly ...

tannase has several important applications in food, feed, chemical and pharmaceutical industries. in the present study, production of tannase by mutant strain, penicillium sp. ez-zh390, was optimized in submerged fermentation utilizing two statistical approaches. at first step, a one factor at a time design was employed to screen the preferable nutriments (carbon and nitrogen sources of the med...

A fungal tannase was produced, recovered, and immobilized by entrapment in calcium alginate beads. Catalytical properties of the immobilized enzyme were compared with those of the free one. Tannase was produced intracellularly by the xerophilic fungus Aspergillus niger GH1 in a submerged fermentation system. Enzyme was recovered by cell disruption and the crude extract was partially purified. T...

Tannase, produced from the fungus Aspergillus flavus by submerged fermentation on different medium was studied. Tannic acid 1 had a higher activity of 70 U/g/min in purified form. The process parameter was optimized and higher production of tannase was found at 35°C and 96 hours of incubation with 2% tannic acid.

Complexing of green tea catechins with food constituents and their hydrolysis by tannase-producing Lactobacillus plantarum strains, were investigated. Our observations indicated that 1) epigallocatechin gallate (EGCg) and other catechin galloyl esters bound with food ingredients (i.e., proteins) to form a complex that is likely to be unabsorbable through the intestinal wall, whereas most catech...

Enzymatic extraction was investigated to improve the quality of black tea extracts with pretreatment of pectinase and tannase independently, successively and simultaneously. Pectinase improved the extractable-solids-yield (ESY) up to 11.5%, without much of an improvement in polyphenols recovery, while tannase pre-treatment showed a significant improvement in polyphenols recovery (14.3%) along w...

Modified solid-state fermentation (MSSF) of tannin-rich substrates for production of tannase and gallic acid was carried out using fungal co-cultures. Tannin acyl hydrolase or tannase is an enzyme produced extracellularly in solid state fermentation by various fungi. The solid-state fermentation offers a number of advantages over submerged and liquid conventional fermentation for the enzyme pro...

Tannin acyl hydrolase also referred as tannase is an enzyme with important applications in several science and technology fields. Due to its hydrolytic and synthetic properties, tannase could be used to reduce the negative effects of tannins in beverages, food, feed, and tannery effluents, for the production of gallic acid from tannin-rich materials, the elucidation of tannin structure, and the...

Naturally immobilized tannase (tannin acyl hydrolase, E.C. 3.1.1.20) has many advantages, as it avoids the expensive and laborious operation of isolation, purification, and immobilization, plus it is highly stable in adverse pH and temperature. However, in the case of cell-associated enzymes, since the enzyme is associated with the biomass, separation of the pure biomass is necessary. However, ...