single-nucleotide polymorphisms (snps) in mirnasmay alter its expression levels or processing and contribute to susceptibility to a wide range of diseases. our study aimed to evaluate the possible association between mirna-146a rs2910164 and mirna-499 rs3746444 polymorphisms and susceptibility to pulmonary tuberculosis (ptb) in a sample of iranian population. this case- control study was perfor...

The C57BLKS/J-Lepr(db) mouse has a point mutation in the leptin receptor gene and is one of the most useful animal model for non-insulin dependent diabetes mellitus in human. Since the homozygote of C57BLKS/J-Lepr(db) mouse is infertile, detection of point mutation in the leptin receptor gene is important for efficient maintaining strains as well as mass production of homozygotes. To develop a ...

Background: Non-coding RNAs apply regulations on expression or function of a gene. A class of non-coding RNAs, natural antisense transcripts, might overlap with their flanking genes and emerge a new complexity upon regulation. WRAP53, is a natural antisense transcript overlapped in a head-to-head manner on the opposite strand of TP53. It has 3 transcripts of which WRAP53β produ...

Potato virus Y biberde enfeksiyon oluşturan en yaygın virüslerden bir tanesidir. Bu çalışmada, 23 biber çeşidi ve 30 ıslah hattının PVY-0 PVY-1 patotiplerine karşı reaksiyonları belirlenmiş pvr2 lokusundaki allel genleri mekanik inokulasyon moleküler yöntemler ile belirlenmeye çalışılmıştır. Her çeşit hatta ait dörder bitki, patotipleri ayrı inokule edilmiştir. İnokule edilen bitkiler, virüs si...

background: bladder cancer (bc) is the most common genitourinary cancer in iranians with an unclear etiopathogenesis. long stress induced non-coding transcript 5 is a member of the 12-member family of long non-coding rnas, which is abundantly expressed in higher proliferative cells, as well as the cell vulnerable to cellular stress in response to chemical carcinogens. as variations in long non-...

BACKGROUND We describe a simple tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) for detecting MUTYH mutations, which are associated with colorectal adenomas and colorectal cancer. METHODS We designed specific T-ARMS-PCR assays for 6 mutations (Y165C, G382D, 1395_7delGGA, Y90X, 1103delC, and R231H) selected on the basis of the frequency of their occurrence. We also desig...

Background & Aims: Tyrosinase is the most important enzyme in the production of pigments of the skin, eyes, and hair follicles. The enzyme is encoded by tyrosinase gene (TYR) or oculocutaneous albinism type 1A (OCA1A). Mutations in TYR gene result in pigmentation disorders such as albinism in humans. In view of the large number of mutations reported in this gene, the aim of this study was to id...

background: bladder cancer (bc) is the most commonly diagnosed genitourinary cancer in iran, presented in both men and women. bc is a multifactorial trait resulting from the complex interaction between several genes and environmental factors. long stress-induced non-coding transcript 5 (lsinct5), a member of the long non-coding rnas, is abundantly expressed in high proliferative cells, as well ...