Objective(s): ISCOMATRIX vaccines have now been shown to induce strong antigen-specific cellular or humoral immune responses to a broad range of antigens of viral, bacterial, parasite or tumor. In the present study, we investigated the role of ISCOMATRIX charge in induction of a Th1 type of immune response and protection against Leishmania major infection in BALB/c mice.  Materials and Methods:...

Objective(s): Despite treatment with antibiotics and vaccination with BCG, tuberculosis (TB) is still considered as one of the most important public health problems in the world. Therefore, designing and producing a more effective vaccine against TB seems urgently. In this study, immunogenicity of a fusion protein which consisting or comprising CFP-10 from Mycobacterium tuberculosis and the Fc-...

Abstract Arginase 1 (ARG1), the enzyme catalyzing conversion of arginine to ornithine and urea, is a hallmark IL-10 producing immunosuppressive M2 macrophages, however ARG1 activity in T cells disputed. Here we demonstrate that Arg1, but not Arg2, expression induction key feature lung CD4 during mouse vivo influenza infection. Ablation cell-intrinsic Arg1 unexpectedly accelerated both virus-spe...

Th1 and Th2 cells mutually antagonize each other's differentiation. Consequently, allergen-specific Th1 cells are believed to be able to suppress the development of Th2 cells and to prevent the development of atopic disorders. To determine whether a pre-existing Ag-specific Th1 response can affect the development of Th2 cells in vivo, we used an immunization model of Ag-pulsed murine dendritic ...

recent studies have proposed that a decline in bacterial infections such as tuberculosis is a factor underlying the rising prevalence and severity of atopic disorder in developed countries. there are conflicting ideas about the inverse relationship between bcg (bacillus calmette-guerin) vaccination and asthma. stronger response to tuberculin test as an indicator of more potent th1 response is s...

Th1 cells, which express IL-18R, produce IFN-gamma in response to Ag and IL-2 and increase further production of IFN-gamma upon additional IL-18 stimulation. They simultaneously produce Th2 cytokines (IL-9 and IL-13), GM-CSF and chemokines (RANTES, MIP-1alpha). Human Th1 cells also produce IFN-gamma and IL-13 in response to anti-CD3 and IL-18. Recently, we demonstrated Th1 cells induce intrinsi...

Experimental systems based on immunization with plasmid DNA or immune-stimulating complexes were used to delineate the requirements for generation of protective immunity against murine leishmaniasis. Vaccination with plasmid DNA encoding the host-protective Leishmania major parasite surface Ag-2 primed for an essentially exclusive Th1 response that protected mice against L. major infection. In ...

We tested the independence hypothesis, namely that the Th1/Th2 nature of concurrent immune responses, generated in the same secondary lymphoid organ to non-cross-reacting Ags, can be independently determined. Some infectious agents and some adjuvants contain modulatory molecules that affect the Th1/Th2 nature of immune responses in a non-Ag-specific manner. We therefore excluded infectious agen...

background: iron deficiency anemia (ida) has been controversially linked to il-4 production in previous studies. a predominant th1 response leads to resistance against recurrent vulvovaginal candidiasis (rvvc), whereas a th2 response exacerbates the disease. objective: to investigate the possible effect of iron deficiency on the host’s susceptibility to rvvc as a result of the th1/th2 cytokine ...