A simple, rapid and precise method that does not involve digestion has been described for determination of plant K. The method involves shaking of 0.5 g of finely ground plant materials with 40 ml of 0.5 N_ HC1 for 5 minutes. The extract is filtered and read in an Atomic absorption spectrophotometer for K. The method gives !< values that are in close agreement with those obtained with conventio...

A highly sensitive and selective high performance liquid chromatographic fluorescence detection method has been developed and validated for the quantification of rivastigmine in rat plasma and brain. Protein precipitation and one-step liquid-liquid extraction techniques were utilized for the extraction of RSM from brain and plasma, respectively, along with an internal standard. The chromatograp...

Background and objectives: According to recent studies, homotaurine may be an Alzheimer's active ingredient. The goal of this project was to extract and detect homotaurine in algae collected from Persian Gulf shores. Methods: Extraction was done with etanol 80% for 30 min in ambient temperature. The HPLC elution solvent required a mixture of two solvents. Solve...

GF-120 is a baited formulation of the insecticide spinosad containing 1% ammonium acetate, developed for control of economically important fruit flies. The response of feral cherry fruit flies, Rhagoletis cingulata Loew, to GF-120 augmented with 0, 5, or 10% ammonium acetate was evaluated under orchard conditions. Significantly more flies were observed within 30 cm of bait droplets with 10% amm...

In this study, we developed and validated a High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS) method to determine bile acid in mouse serum. The serum samples were analysed after solid-phase extraction. The analytes were separated on a Diamonsil C18 column with a mobile phase of methanol and water containing 10 mmol/L ammonium acetate and 0.005% formic acid (70:30) at ...

A rapid and highly sensitive liquid chromatography/tandem mass spectrometric (LC/MS/MS) method for determination allopurinol (AP) in human plasma was developed. The analyte and internal standard (IS), Ganciclovir-d5, were extracted from human plasma via liquid/liquid extraction (LLE) with ethyl acetate (2.5mL), supernatant was dried and reconstituted in 200 μL of 10mM ammonium acetate-methanol ...

This Application Note describes an HPLC/DAD method for the simultaneous determination of methotrexate (MTX) and sulfasalazine (SSZ) in plasma using an Agilent 1290 Infinity LC System. MTX and SSZ are used in combination for the treatment of rheumatoid arthritis. Using two different detector wavelengths, 304 nm for MTX and 358 nm for SSZ, we were able to selectively quantitate both analytes duri...

Ammonia is the primary attractant for tephritid fruit flies, and traps baited with synthetic attractants using ammonia formulations have been highly successful in capturing these pests. However, difficulties in quantifying release rates of ammonia have limited abilities to make comparisons among field tests of different species by using different formulations. Therefore, Fourier transform infra...