OBJECTIVE To compare the outcome of transfer of thawed blastocysts frozen on either day 5 or day 6 after in vitro fertilisation. DESIGN Cohort observational study. SETTING Tertiary assisted conception unit in London, UK. POPULATION Six hundred and forty-two consecutive nondonor programmed thawed blastocyst transfer (TBT) cycles. METHODS High-grade blastocysts were frozen on day 5 (n = 3...

Regulations concerning the storage of transfusable plasma differ internationally. In Canada, plasma obtained from whole blood donations and frozen within 24 hours of phlebotomy (frozen plasma, FP) may be thawed and transfused within 120 hours of refrigerated storage. However, plasma frozen within 8 hours of phlebotomy following apheresis donation (FFPA) must be transfused within 24 hours of tha...

BACKGROUND Artificial insemination (AI) using frozen-thawed semen is well established and routinely used for breeding in various mammalian species. However, there is no report of the birth of elephant calves following AI with frozen-thawed semen. The objective of the present study was to investigate the fertilizing ability of chilled and frozen-thawed semen in the Asian elephant following artif...

OBJECTIVE To compare the fat loss between refrigerator and warm water thawed breast milk. DESIGN Experimental. SETTING Tertiary-care pediatric university hospital. PARTICIPANTS Ninety samples of expressed breast milk were collected from mothers with singleton babies of a gestational age 32-42 weeks. MAIN OUTCOME MEASURES Fat content in fresh breast milk (FM); thawed breast milk by refri...

The influence of thawing procedures on fertility of semen in .25-ml Continental 3 straws was investigated in a series of eight trials utilizing 1,634 cows. The following pregnancy rates were obtained: Trial 1, 38.6 vs 23.0% (P<.05) for semen thawed in 75 C water/7 sec vs "in the cow"; Trial 2, 29.7 vs 22.2% (P>.10) for semen thawed in 75 C water/7 sec vs "in the cow"; Trial 3, 82.8 vs 61.6% (P<...

The advantages of lipid-based transfection reagents have permitted their widespread use in molecular biology and gene therapy. This study outlines the effect of cryo-manipulation of a cationic lipid-based formulation, Lipofectamine 2000, which, after being frozen and thawed, showed orders of magnitude higher plasmid delivery efficiency throughout eight different cell lines, without compromising...

The ability of the fetal pancreatic islet cells to multiply rendered them a potential tissue for transplantation studies to cure diabetes. A bank of fetal islets could be created with proper storage in liquid nitrogen. The aim of this study is to evaluate the effect of thawing rate and post-thaw culture on the structural and functional integrity of isolated cryopreserved islets of rat fetuses. ...

This retrospective consecutive case series aimed at comparing the results of intracytoplasmic sperm injection (ICSI) with fresh and with frozen-thawed epididymal spermatozoa obtained after microsurgical epididymal sperm aspiration (MESA) in 162 couples. These couples were suffering from infertility because of congenital bilateral absence of the vas deferens (n = 109), failed microsurgical rever...

Post-thawed larval rearing in Pacific oyster Crassostrea gigas was performed to investigate the survival rate with time course in three kinds of larvae cryopreserved. The highest survival rate and larval activity index (LAI) of post-thawed larvae were obtained from the permeation in 0.2 M sucrose and 2.0 M ethylene glycol (EG) at -1°C/min in freezing speed showing the survival rates just after ...

OBJECTIVE To evaluate if the outcomes of IVF/ICSI in frozen-thawed embryo transfer and fresh embryo transfer cycles differ in relation to cleavage and blastocyst stages. METHODS Retrospective cohort study to compare IVF/ICSI outcomes between fresh embryo transfer and frozen-thawed embryo transfer cycles, according to the stage of embryo development. Analysis was carried out on 443 consecutive...