OBJECTIVES To identify the major allergenic proteins of clam (Paphia textile) and to investigate the effect of different cooking methods on the allergenicity of these identified proteins. METHODS Clam protein extracts were separated by denaturing polyacrylamide gel electrophoresis. IgE reactive proteins were then analyzed by immunoblotting with sera from patients with positive skin prick test...

The psychrophile Clostridium sp. strain 69 contains a thermolabile triose phosphate isomerase which is inactivated rapidly in vitro and in vivo at 32 C.

Background: The role of food allergy in atopic dermatitis (AD) is controversial. The aim of this study was to determine the frequency of food allergens in children with AD. Method: Fifty one atopic dermatitis children aged 6 months to 5 years entered our study. The severity of AD was determined by scoring index of AD (SCORAD). Skin prick test (SPT) was done using 15 allergens including the whit...

Background: Several studies reported the clinical features of IgE-mediated hypersensitivity after ingestion of melon. Melon allergy is a common IgE-mediated fruit allergy in Iran. This prompted us to investigate immunochemical and molecular properties of the major allergen in melon fruit, to compare the IgE-binding capacity of the natural protein with the recombinant allergen, and to determine ...

The efficacy of allergen immunotherapy for the treatment of allergic rhinoconjunctivitis with or without seasonal bronchial asthma and anaphylaxis caused by the sting of the hymenoptera class of insects has been clearly demonstrated in numerous well-designed, placebo-controlled trials. Immunotherapy whether by subcutaneous injection of allergen extract or by oral/sublingual routes modifies peri...

Stanziale, W. G. (Fort Detrick, Frederick, Md.) and J. D. White. Influence of guinea pig plasma factors on phagocytosis of Pasteurella pestis. I. Plasma from normal guinea pigs. J. Bacteriol. 83:175-181. 1962.-The existence of an enhancing effect of normal guinea pig plasma on the phagocytosis of Pasteurella pestis has been reaffirmed, using a test system consisting of neutrophils from normal n...

BACKGROUND Raised levels of total plasma homocysteine (tHcy) are associated with an increased risk of retinal vascular occlusive disease. A thermolabile form of a pivotal enzyme in homocysteine metabolism, methylenetetrahydrofolate reductase (MTHFR), has been associated with vascular occlusive disease and raised tHcy levels. The relation between thermolabile MTHFR genotype, tHcy, and retinal va...

specific local immunotherapy has been recently introduced as an alternative to classic subcutaneous immunotherapy in treatment of allergic rhinitis. in this study, the effects of sublingual immunotherapy (slit) on symptoms and medication score and skin prick test evaluation of patients with allergic rhinitis were investigated. in this placebo controlled trial, twenty four patients aged 5-18 yea...

RNase H of a temperature-sensitive mutant of Rauscher murine leukemia virus is thermolabile, establishing this activity as a virus-coded function of the mammalian type C virus reverse transcriptase.