Pectinase was produced by solidstate fermentation utilizing coffee pulp with Aspergillus niger CFR 305. The exogenous pectinase contained 7539 ±70 U/g of enzyme activity. Further the pectinase enzyme was partially decolorized with activated carbon and 80 % recovery of enzyme activity was obtained. The optimum pH and temperature of the crude decolorized pectinase was 4.5 and 50°C respectively. T...

In this paper, the saccharification process of okara fiber with pectinase, xylanase and cellulase was investigated as a preliminary attempt to effectively utilize okara fiber. The solubilization of okara fiber was raised in proportion to the number of enzymes used; that is, by a single enzyme, two enzymes and three enzymes, in that order. The saccharification of okara fiber was much more comple...

In this work, the effect of particle size on the enzymatic hydrolysis of milled and sieved sugarcane bagasse (SCB) was studied. The enzymatic hydrolysis and fermentability of superfine ground SCB (SGP400) using an enzyme cocktail strategy were also explored. Particle size reduction improved the enzymatic hydrolysis. The highest glucose yield was 44.75%, which was obtained from SGP400. The enzym...

Plant peels could be a potential source of novel pectinases for use in various industrial applications due to their broad substrate specificity with high stability under extreme conditions. Therefore, the extraction conditions of a novel pectinase enzyme from pitaya peel was optimized in this study. The effect of extraction variables, namely buffer to sample ratio (2:1 to 8:1, X₁), extraction t...

digestibility in broiler chickens fed on corn-soybean meal based diets. the experimental diets consisted of 1.corn-soybean meal based control diet 2. control diet supplemented with pectinas, 3. control diet supplemented with cellulase, 4. control diet supplemented with pectinase + cellulose, 5. control diet supplemented with pectinase + hemicellulase and 6. control diet supplemented with pectin...

A thermophilic fungal strain producing both pectinase and polygalacturonase was isolated after primary screening of 120 different isolates. The fungus was identified as Aspergillus fumigatus Fres. MTCC 4163. Using solid-state cultivation, the optimum levels of variables for pectinase and polygalacturonase (PG) production were determined. Maximal levels of enzyme activities were achieved upon gr...

Enzymatic activity e.g., pectinase, cellulase, protease and lipase of different fungal pathogens causing stalk rot disease in corn was determined. In-vitro studies; corn stalk rot pathogens viz., Cephalosporium acremonium, Fusarium moniliforme, F. graminearum, F. semitectum, Macrophomina phaseolina, st. 1, M. phaseolina st. 2, Rhizoctonia solani st.1, R. solani st. 2 and Verticillium albo-atrum...

A novel strain Rhizopus sp. C4 was isolated from compost for the production of pectinase. Cultivation of Rhizopus sp. C4 on orange peel substrate under various solid-state fermentation (SSF) conditions was evaluated for pectinase yield along with the enzyme activity profile as a potential, low-cost alternative to submerged-liquid fermentation. Response surface methodology (RSM) was employed to ...

The use of enzyme could facilitate pathogen penetration into plant host. Here the combination of cellulase and pectinase was ascertained on the pathogenicity of F. compactum (1.4 × 10(6)  propagules ml(-1)) on broomrape tubercles. F. compactum alone infected all the inoculated tubercles but did not kill any significant number. Infested tomato roots that were inoculated with mycelia plus pectina...

Orange bagasse comprising pulp tissues, rind, and seeds, constitutes a major industrial food waste arising from processing oranges for juice, and represents a fermentation feedstock for the production of enzymes. Botryosphaeria rhodina MAMB-05 grown on essential oils-extracted orange bagasse in submerged (SmF) and solid-state fermentation (SSF) with and without added nutrients produced pectinas...