Polyploidy was induced in Melia volkensii (Mukau), a valuable native tree from the semi-arid regions of East Africa. Cotyledons diploid M. (2n = 2x 28) were treated with oryzalin for 0 (control), 1, 2, or 3 h without pretreatment 1.1 µM thidiazuron. 10 mg·L−1 three hours yielded 40% tetraploids. Pretreatment cotyledons thidiazuron 18 days followed by treatment increased tetraploid plant product...

An in vitro method was developed for high frequency shoot regeneration and continuous production of Brassica napus L. in large number. The different concentrations of benzyladenine (1.5, 3.0 and 4.5 mgl and thidiazuron (0, 0.15 and 0.30 mgl) were evaluated for shoot and root regeneration using hypocotyl explants with 7, 14 and 21 days old. Shoot regeneration (SR) was affected by both benzyladen...

Malik A.A., Li Cui, Shuxia Zhang, Jin-feng Chen, 2011. Efficiency of SSR markers for determining the origin of melon plantlets derived through unfertilized ovary culture. Hort. Sci. (Prague), 38: 27–34. The effects of temperature pre-treatment, thidiazuron, naphthaleneacetic acid, and 6-benzylaminopurine on in vitro gynogenic plant production from un-pollinated melon (Cucumis melo L.) ovaries w...

Glucosides of trans-zeatin occur widely in plant tissues, formed either by O-glucosylation of the hydroxylated side chain or N-glucosylation of the purine ring structure. O-Glucosylation is stereo-specific: the O-glucosyltransferase encoded by the Phaseolus lunatus ZOG1 gene has high affinity for trans-zeatin as the substrate, whereas the enzyme encoded by the maize (Zea mays) cisZOG1 gene pref...

In vitro seedlings were used as explants for protocorm like bodies (PLBs) production which in turn were used for regeneration purpose. PLBs were induced from the base of seedlings (1.0-1.5 cm in size) in MS + BAP (8.88 microM). After 90 days of inoculation, PLBs production rate started declining and most of the PLBs turned into plantlets. Preculture of seedlings in 1.0 microM thidiazuron (TDZ) ...

Hybrids of Solanum melongena and S. aethiopicum are of interest as rootstocks of eggplant, as they are highly vigorous and can incorporate resistance to several diseases. However, hybridization between both species is difficult. Therefore, protocols for in vitro culture are of great interest for their micropropagation and biotechnological breeding. We assessed the organogenesis response from le...

Sprouted corm sections of "South Dade" white cocoyam were potted and maintained in a greenhouse for 8 weeks. Shoot tips of 3-5 mm comprising the apical meristem with 4-6 leaf primordial, and approximately 0.5 mm of corm tissue at the base. These explants were treated to be used into the culture medium. A modified Gamborg's B5 mineral salts supplemented with 0.05 μM 1-naphthaleneacetic acid (NAA...

The marketability of cut flowers relies on its postharvest longevity, making it crucial to develop technologies that canextend vase life. life in chrysanthemum is dependent upon floret senescence and leaf yellowing. In view ofextending the life, present study elucidates implication plant growth regulators viz. benzyl adenine (BA),thidiazuron (TDZ) salicylic acid (SA) performance flower longevit...