A unidimensional Thin Layer Chromatography system for the separation of salicylic acid and its derivatives (sulfosalicylic acid and Aspirin) has been developed. The separation was successfully using a solvent system of chloroform-methanol-water-ammonium hydroxide 120:75:6:2 (V/V) as mobile phase on TLC plates impregnated with boric acid. The method is based on different behavior of investigated...

In the view of high birth rate, a large burden of preventable mental retardation in the form of aminoaciduria occurs in developing country like India. While neonatal screening by tandem mass spectrometry (TMS) and HPLC (High Pressure Liquid Chromatography) is almost universal in developed countries but scarce in India due to high cost. Hence, this study was planned to asses thin layer chromatog...

Thin layer chromatography (TLC) stained with hot acidic p-anisaldehyde, is an interesting, fast, and low-cost technique to monitor main lipid contaminants such as triacylglycerols, diacylglycerols, and monoacylglycerols in biodiesel. These acylglycerols are detectable by the proposed planar chromatographic method, provided the content of the contaminants exceeds the limits recommended by the in...

There has been a tendency to discount thin-layer chromatography (TLC) in recent years because of the availability of high-performance liquid chromatography (HPLC) methods that appeared to offer greater versatility and ease of quantification, amongst other reasons. However, TLC has made a comeback, largely because of the high reproducibility and lower costs of commercial pre-coated plates. It is...

In the previous paper from this series, we proposed mass spectrometric fingerprinting of complex botanical samples upon the examples of the pharmacologically important phenolic acids and flavonoids selectively extracted from Salvia lavandulifolia. In this study, we explore fingerprinting efficiency with a novel two-dimensional analytical system composed of the reversed-phase thin-layer chromato...

This study describes a method for off-line coupling of thin-layer chromatography (TLC) with electron impact ionization (El)-mass spectrometry for routine determination of pesticides in toxicology and forensic medicine. Six TLC solvent systems are described for 151 different pesticides, and 8-peak mass-spectra generated from full El mass spectra are listed.

Thin layer chromatography (TLC) is routinely used by synthetic organic chemists and natural products chemists working on a variety of types of molecules. Carbohydrate chemists and biochemists in the past largely relied on paper chromatography for rapid and inexpensive analysis. Carbohydrates are highly polar molecules and often require derivatization to be analyzed by TLC. Acidic carbohydrates ...

Objective: To establish the quality standard for Penyankang Suppository. Method: Thin Layer Identification (TLC) was used for the qualitative identification of Caulis Sargentodoxae, Radix Salviae Miltiorrhizae, Rhizoma Corydalis and Rhizom Sparganii. High performance liquid chromatography (HPLC) was used to determine the contents of salvianolic acid B and tetrahydropalmatine. Results: Thin Laye...

The isolation and identification of the phosphonolipids from beef brain--right lobe, left lobe and cerebellum--is herein reported. The phosphonolipids were isolated by preparative thin layer chromatography (TLC) using the solvent system methanol/water (2:1, v/v) and identified preliminarily by TLC. The isolated phosphonolipids were subjected to silicic acid column chromatographic separation and...