BACKGROUND Although much is known about the reactivity of polyclonal populations of antibodies targeting the wide array of allergens produced by timothy (Phleum pratense) and other grass species, little is known about the finer details at the level of individual antibody specificities. OBJECTIVE We sought to investigate the IgE repertoire as it occurs in a patient with grass pollen allergy. ...

BACKGROUND Allergen extracts can degrade when exposed to temperatures significantly beyond the optimum storage recommendation of 4 degrees C. Many allergen extracts are mailed to their final destinations throughout the year with exposure to varied environmental conditions. OBJECTIVE To evaluate the effect of summer mailing on the in vitro and in vivo potency of timothy grass extract. METHOD...

BACKGROUND Immunological test systems for diagnostics of type I hypersensitivity involve the following types of antigens: whole allergen extracts, individual highly purified proteins and their recombinant analogues. The goal of this study was to compare the results obtained with whole allergen extracts (birch pollen, cat dander, and timothy grass pollen) and their respective recombinant protein...

BACKGROUND Many studies have reported the prevalence of sensitization using skin prick tests. However, comparisons between studies and between regions are difficult because the number and the type of allergens tested vary widely. Using the European Community Health Respiratory Survey I data, the geographical variation of sensitization to environmental allergen as measured by skin tests was esta...

Caldicellulosiruptor bescii efficiently degrades cellulose, xylan, and native grasses at high temperatures above 70°C under anaerobic conditions. C. bescii extracellularly secretes multidomain glycoside hydrolases along with proteins of unknown function. In this study, we analyzed the C. bescii proteins that bind to the cell walls of timothy grass by using mass spectrometry, and we identified f...

Most of the 400 million grass pollen-allergic patients worldwide are co-sensitized to several unrelated grass pollen allergens. Based on frequent co-sensitization patterns determined in 200 grass pollen-allergic patients, three recombinant hybrid molecules were developed by polymerase chain reaction-based mending of cDNAs coding for the major timothy grass pollen allergens (Phl p 1, Phl p 2, Ph...

Abstract Nowadays due of the world population growth, demand for agricultural products is also growing. To obtain good meat and dairy products, high-quality feed farm animals necessary. Legume-gramineous perennial grass mixtures are used to improve fodder lands. Our experience included a test 9 variants legume-gramineous mixtures, where 6 types grasses were used: red clover, alfalfa (legumes), ...