background: we have previously reported that a 31 kda protein was screened from the excretory-secretory (es) proteins of tichinella spiralis muscle larvae (ml) by immunoproteomics using early infection sera, and the gene encoding a 31 kda protein from t. spiralis was cloned and expressed in an e. coli expression system. in this study, the recombinant 31 kda antigens were used for detection of a...

A reverse line blot (RLB) assay was developed to identify different Trichinella genotypes. The RLB assay accomplishes detection and specific identification of the different Trichinella genotypes and relies on hybridization of the amplified 5S ribosomal DNA intergenic spacer regions to specific, membrane-bound oligonucleotide probes. After one single amplification, we were able to detect and gen...

Trichinellosis is a globally important food-borne parasitic disease of humans caused by roundworms of the Trichinella complex. Extensive biological diversity is reflected in substantial ecological and genetic variability within and among Trichinella taxa, and major controversy surrounds the systematics of this complex. Here we report the sequencing and assembly of 16 draft genomes representing ...

CRANDALL, RICHARD B., AND CRANDALL, CATHERIXE 4. 1972. Trichinella spiralis: Immunologic response to infection in mice. Experimen~tnl Purasitology 31, 378-398. Serum IgG1, IgG2. IgM, and IgA levels were measured by radial immunodiffusion in C57B1 mice after infection with 200 Trichinella larvae. IgG, concentrations increased 2to 5-fold during t,he second week of infection; IgA and IgGz levels s...

b ackground: the objective of this investigation was to detect the presence of trichinella in some carnivores of mashhad in northeast of iran and to identify trichinella species circulating in this area. methods: the present study was carried out using muscle tissue collected from 120 stray dogs, 26 wild boars, 25 rodents, two foxes and two hyenas captured in mashhad city, province of khorasan ...

Human and animal trichinellosis should be considered as both an emerging and reemerging disease. The reemergence of the domestic cycle has been due to an increased prevalence of Trichinella spiralis, which has been primarily related to a breakdown of government veterinary services and state farms (e.g., in countries of the former USSR, Bulgaria, Romania), economic problems and war (e.g., in cou...

The first outbreak of human trichinellosis in China,according to official national publication,was registered in Tibet in 1964. Since then,more than 500 outbreaks, numbering 25 161 cases with 240 deaths, have been recorded.However, this reported quantity is probably highly underestimated because adequate diagnostic techniques might not have been available in the whole area.Moreover, infection i...

In order to study the epidemiology of trichinellosis with special emphasis on swine a detailed study was performed. The goals of the study were to define the prevalence of trichinellosis in swine and rats and to determine the etiological agents of the disease with special emphasis on sylvatic species, Trichinella britovi. Considering the obtained data it can be concluded that the risk of Trichi...

Since 1995, Trichinella larvae have been detected in 39.5% of farmed crocodiles (Crocodylus niloticus) in Zimbabwe. Morphological, biological, biochemical and molecular studies carried out on one isolate from a farmed crocodile in 2001 support the conclusion that this parasite belongs to a new species, which has been named Trichinella zimbabwensis n.sp. This species, whose larvae are non-encaps...