We demonstrate a rapid cloning and sequencing strategy for kilobase-size DNA segments using DNase I and long PCR. In a single-tube protocol, deletions were formed in a plasmid insert by two enzymatic cuts, one at a fixed site and one at random. The doubly cut molecules were recircularized to generate a library of plasmids carrying deletions of various sizes and transformed into E. coli. The pla...

OBJECTIVE To describe safety, tolerability, and effectiveness results through 5 years of follow-up of a Phase III trial with Essure inserts. DESIGN Multicenter, nonrandomized, single-arm international study (Canadian Task Force classification II-3). SETTING Thirteen clinical study centers in the United States, Europe, and Australia. PATIENTS A total of 518 previously fertile women seeking...

Numerical simulations and experiments are carried out in this paper to research about characteristics of heat transfer, frictional resistance comprehensive performance turbulent water flow (3000 < Re 10,000) a circular tube with twisted tape inserts. A new type is proposed it shows excellent on transfer enhancement. Effects three parameters including twist ratio (p/w=3, 4, 5), geometrical struc...

One of the disadvantages of conventional milling tools is to produce a large amount of heat in cutting zone and so making the tool warmer. As a result, the tool would be worn and its life time would be reduced. Therefore, in long time machining, it is essential to change inserts and tools, continiously. A milling tool with self rotary cutting inserts was designed and manufactured that enables i...

In this study, the heat transfer rate of Al2O3-water nanofluid-flow in a tube with twisted tape inserts is experimentally examined. The insert can cause swirling region fluid-flow which will enhance coefficient. On other hand, have great influence on pressure drop. It known that nanoparticles and may both increase fluid. Thus, it tried to demonstrate combined effect phenomena, experimentally. w...

Five wire-coil inserts with fixed wire diameter and different pitches, fitted inside a round tube have been experimentally studied in transitional low turbulent flow. Water was used as working fluid at wide range of flow conditions: 103 &lt; Re 104 3.9 Pr 10.0. The geometrical parameters the are: e/Di = 0.070, p/e 6.7, 9.0, 10.0, 12.5, 15.0. variation friction factor heat transfer coefficients ...

There is a growing appreciation of the potential value for routine screening for the presence of HPV not only for cervical specimens but also from oral cavity. The purpose of this study was to develop and clinically evaluate a single-tube seminested PCR assay for the detection of HPV. Several parameters such as PCR primers, primer annealing temperature, the number of PCR cycles and concentratio...