Microtubules are essential for various cellular activities and β-tubulins are the target of benzimidazole fungicides. However, the evolution and molecular mechanisms driving functional diversification in fungal tubulins are not clear. In this study, we systematically identified tubulin genes from 59 representative fungi across the fungal kingdom. Phylogenetic analysis showed that α-/β-tubulin g...

To investigate the prevalence of fungal agents in local Egyptian broiler breeder chicken’s premises, tracheal and cloacal swabs from chickens, feed, and water samples were collected. The targeted breed's dead-in-shell eggs and newly hatched chick’s samples were also tested. All fungal isolates were morphologically typed and the predominant fungal species were further subjected to molecular typi...

More than 20 years ago, biochemical analysis of the eukaryotic cell cytoskeleton revealed the major component proteins. The heterodimeric (α/β) protein tubulin was defined as the building block of microtubules, assembled in a polar manner into specifically arranged protofilaments in the microtubule wall [1]. The next two members of the tubulin protein superfamily were both discovered by genetic...

objective: mesenchymal stem cells (mscs) can differentiate into various cell types. since cysteine has structural similarities to neuronal inducers β-mercaptoethanol and glutathione, we examined its effect on neural induction of rat bone marrow mscs. materials and methods: in this experimental study, cells were treated in a medium containing 1mm cysteine for 24 hours prior to treatment with neu...

Chrysanthemum is an important flower crop grown in different parts of the world. Apparently there are new reports chrysanthemum collar rot and root caused by Phytophthora sp. around In recent years has been increase incidence no information available about this disease India. Therefore aim present study to isolate characterize pathogen causing chrysanthemum. During year 2021, plants exhibiting ...

As a major component of the cytoskeleton, microtubules consist of αβ-tubulin heterodimers and have been recognized as attractive targets for cancer chemotherapy. Microtubule-stabilizing agents (MSAs) promote polymerization of tubulin and stabilize the polymer, preventing depolymerization. The molecular mechanisms by which MSAs stabilize microtubules remain elusive. Here we report a 2.05 Å cryst...

The structure of the unique bacterial tubulin BtubA/B from Prosthecobacter is very similar to eukaryotic αβ-tubulin but, strikingly, BtubA/B fold without eukaryotic chaperones. Our sequence comparisons indicate that BtubA and BtubB do not really correspond to either α- or β-tubulin but have mosaic sequences with intertwining features from both. Their nucleotide-binding loops are more conserved,...

The study investigated the clinical significance of RRM1 (ribonucleoside reductase subunit M1), TUBB3 (tubulin-β-III), TOP2A (DNA topoisomerase II), CYP19A1 (cytochrome P450, family 19, subfamily A, polypeptide 1) and CYP2D6 (cytochrome P450, family 2, subfamily D, polypeptide 6) for the diagnosis and possible predictive roles in breast cancer. Tissue microarray detected the expression of RRM1,...

Ninefold microtubule symmetry of the eukaryotic basal body and motile axoneme has been long established [1–3]. In Drosophila, these organelles contain distinct but similar β-tubulin isoforms [4–10]: basal bodies contain only β1-tubulin, and only β2-tubulin is used for assembly of sperm axonemes. A single α-tubulin functions throughout spermatogenesis [11,12]. Thus, differences in organelle asse...

Autoimmune inner ear disease is described as progressive, bilateral although asymmetric, sensorineural hearing loss and can be improved by immunosuppressive therapy. We showed that the inner ear autoantigen β-tubulin is capable of inducing experimental autoimmune hearing loss (EAHL) in mice. Immunization of BALB/c mice with β-tubulin resulted in hair cell loss and hearing loss, effects that wer...