Two-dimensional (2D) polyacrylamide gel electrophoresis is a classical technique for the separation of proteins. It Rrst appeared in the mid-1970s but for a long time it only found limited applications. Recently it has enjoyed an impressive renaissance. The major reasons for this are the introduction of the immobilized pH gradient (IPG) strips and the development of analytical methods capable o...

In this paper, the two-dimensional triangular orthogonal functions (2D-TFs) are applied for solving a class of nonlinear two-dimensional Volterra integral equations. 2D-TFs method transforms these integral equations into a system of linear algebraic equations. The high accuracy of this method is verified through a numerical example and comparison of the results with the other numerical methods.

We compared cellular proteins from normal human blood lymphocytes, monocytes, and granulocytes, using highresolution two-dimensional electrophoresis. The leukocytes were isolated from peripheral blood by centrifugation on density step gradients (yielding a fraction of purified granulocytes and a lymphocyte/monocyte mixture) and monocytes were subsequently separated from lymphocytes by virtue of...

The mitochondrial polypeptides from maize lines susceptible and resistant to Helminthosporium maydis were studied by single- and two-dimensional gel electrophoresis. Approximately 120 polypeptides were detected by two-dimensional gel electrophoresis. However, it was not possible to detect qualitative differences between the mitochondrial polypeptides of the two inbreds. These observations are d...

Consistent differences in expression of specific proteins were observed between numerous cancer cell clones with high and low potential for spontaneous metastasis. Seventeen clones from two unrelated murine fibrosarcomas were examined concomitantly for spontaneous formation of lung metastases and for occurrence of individual polypeptide differences by high resolution two-dimensional gel electro...

We developed maximum clique-based algorithms for spot matching for two-dimensional gel electrophoresis images, protein structure alignment and protein side-chain packing, where these problems are known to be NP-hard. Algorithms based on direct reductions to the maximum clique can find optimal solutions for instances of size (the number of points or residues) up to 50-150 using a standard PC. We...