نتایج جستجو برای: universal primers
تعداد نتایج: 133448 فیلتر نتایج به سال:
The polymerase chain reaction (PCR) is one of the most rapidly expanding methods in molecular biology. Although the range of applications of PCR is very broad, the key points for successful performance remain the careful selection of optimal primers and the proper determination of the temperature conditions of the reaction. The program presented here, 'Primer Master', has been developed to assi...
New bioactive proteins need to be screened from various microorganisms for the increasing need for industrial and pharmaceutical peptide, proteins, or enzymes. A novel polymerase chain reaction (PCR) method, restriction site-dependent PCR (RSD-PCR), was designed for rapid new genes cloning from genomic DNA. RSD-PCR strategy is based on these principles: (i) restriction sites disperse throughout...
High throughput sequencing technology has great promise for biodiversity studies. However, an underlying assumption is that the primers used in these studies are universal for the prokaryotic or eukaryotic groups of interest. Full primer universality is difficult or impossible to achieve and studies using different primer sets make biodiversity comparisons problematic. The aim of this study was...
DNA barcoding is the use of short DNA sequences (~650 bp) of the standard segment of the genome for large scale species identification. The Consortium for the Barcode of Life (CBOL) plant-working group recommended the 2-locus combination of rbcL and matK as the standard plant barcode. These two regions of chloroplast DNA were chosen due to efficient recovery of quality sequences and high levels...
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