DNA adenine methyltransferase (Dam) not only regulates basic cellular functions but also interferes with the proper expression of virulence factors in various pathogens. We showed previously that for the human pathogen Yersinia enterocolitica, overproduction of Dam results in increased invasion of epithelial cells. Since invasion and motility are coordinately regulated in Y. enterocolitica, we ...

Herein, using DNA adenine methylation (Dam) methyltransferase (MTase) as a model analyte, a novel fluorescence sensing strategy was developed for facile, rapid and highly sensitive detection of the activity and inhibition of the target based on methylation-blocked enzymatic recycling amplification. In this sensing system, nicking endonuclease Nt.AlwI with the methylation-sensitive property was ...

The main purpose of the dam is to control the surface streams and rivers across the country. Dam construction and formation of river and big water reservoirs and resources happen in the glen is a big incident which would change its surrounding area considerably. In fact, constructing a dam the glen width is close and fishes don't migrate from upstream to downstream and ultimately it would led t...

farmers in the indus basin, pakistan have generally switched to groundwater for additional water supplies due to the irregular supply of irrigation water; currently over 50% of the agricultural land in the basin is at least partially irrigated by tube-wells. these wells pump fresh groundwater, which essentially is the result of massive leakage from irrigation canals into the originally saltwate...

the contents of as, cd, cu and mo were determined in soil surrounding meyduk tailings dam based on 21 surface soil samples. assessment of toxic element pollution in studied soil samples needs knowledge of pre-anthropogenic metal concentration to act as a reference against which measured values could be compared. estimating the background values was executed by sampling rocks adjacent to soil sa...

The Au nanorod (AuNR) assembly-enhanced surface plasmon resonance (SPR) system coupling with polymerization and nicking reactions was developed for amplified detection of DNA methylation and adenine methylation (Dam) methyltransferase (MTase) activity assay. The biosensor showed a good linear relationship between the SPR angle shift and the Dam MTase concentration over a range from 0.5 to 120 U...

constructionof underground dams is a practical solution to save groundwater in alluvium watersheds, particularly in arid and semi-arid regions where surface water scarcity is an environmental challenge. considering socio-economic and environmental benefits of underground dams, the accurate locating is the primary consideration for dam construction. the new technologies and methods are a step to...

Seismic methods were used to locate an impermeable water barrier (density body), constructed in front of a homogenous earth-fill dam (Prätisbach) in Eastern Styria, Austria. This detention reservoir, built in 1985/86, was constructed of locally mined materials. Key to this structures functionality is its impermeable core located within the upstream toe of the dam and parallel to the dam axis. T...

This study aims to provide a rapid screening tool for assessment of sustainable flood retention basins (SFRBs) to predict corresponding dam failure risks. A rapid expert-based assessment method for dam failure of SFRB supported by an artificial neural network (ANN) model has been presented. Flood storage was assessed for 110 SFRB and the corresponding Dam Failure Riskwas evaluated for all dams ...