A mutant, USDA 206C, of Rhizobium fredii USDA 206 was obtained by passage on acridine plates. This mutant was cured of its 197-megadalton Sym plasmid but retained its symbiotic effectiveness. Multiple plasmid and chromosomally borne nif gene copies have previously been shown in R. fredii USDA 206. HindIII and EcoRI restriction enzyme digests of plasmid and total DNA showed that at least two nif...

In order to assess the physiological responses of bradyrhizobia and competition for the nodulation of soybean at different temperatures, we investigated the expression of the nodC gene at 20, 25, and 30°C and the abilities of bacteria to nodulate soybean in microcosms at day/night cultivation temperatures of 23/18°C, 28/23°C, and 33/28°C for 16/8 h. We tested five Bradyrhizobium USDA strains: B...

Hypoglycemia represents a dangerous acute condition for neonates and fordiabetics of all ages. For neonates, careful monitoring of plasma glucoseconcentrations in at-risk newborns over the first few hours of life has becomestandard medical practice throughout the nation to guard against hypoglycemia.However, today’s state-of-the-art techniques for accurately detectinghypogly...

BACKGROUND The USDA snack food and beverage standards take effect in school year (SY) 2014-2015. Although the USDA standards will provide nationwide requirements, concerns exist about compliance. This study examined whether existing state laws are aligned with the USDA standards to determine whether some states may be better positioned to facilitate compliance. METHODS Codified state statutor...

Jesse Poland, PI, USDA-ARS, Kansas State University; Kevin Price, CoPI, KSU; Michael Gore, Collaborator, USDA-ARS; Pedro Andrade-Sanchez, Collaborator, University of Arizona; Allan Fritz, Collaborator, KSU; Randy Price, Collaborator, KSU; Jeff White, Collaborator, USDA-ARS; Andrew French, Collaborator, USDA-ARS; Kelly Thorp, Collaborator, USDAARS; William Schapaugh, Collaborator, KSU; Stephen W...