نتایج جستجو برای: using commercial kit and rnease mini kit
تعداد نتایج: 17345105 فیلتر نتایج به سال:
OBJECTIVES The evaluation of Mutans streptococci (MS) is one of the index for caries risk. Dentocult(TM) and CRT(TM) are commercial kits to detect and evaluate MS, conveniently. However, the evaluation of MS has also been carried out simply using an instruction manual. But the instruction manual is not easy to use for evaluation of MS. The aim of this study was to examine the utility of modifie...
AIM Discussing a protocol involving xylene-ethanol deparaffinization on slides followed by a kit-based extraction that allows for the extraction of high quality DNA from FFPE tissues. METHODS DNA was extracted from the FFPE tissues of 16 randomly selected blocks. Methods involving deparaffinization on slides or tubes, enzyme digestion overnight or for 72 hours and isolation using phenol chlor...
The extraction and further processing of nucleic acids (NA) from formalin-fixed paraffin-embedded (FFPE) tissues for microbiological diagnostic polymerase chain reaction (PCR) approaches is challenging. Here, we assessed the effects of five different commercially available nucleic acid extraction kits on the results of real-time PCR. FFPE samples from organs of Burkholderia pseudomallei-infecte...
Introduction: Helicobacter pylori, as an etiologic cause of peptic ulcers and gastric cancer, should be tested and treated. The true state of infection can only be detected by isolation of gastric biopsies through the invasive method of gastroscopy. However, there are several non-invasive methods for detection of infection, the most common of which is serology. Methods: Here we have evaluated t...
عنوان بررسی بروز پروتئین c-kit (cd117) در چهار تومور خوش خیم و بدخیم شایع غدد بزاقی مقدمه و هدف پلئومورفیک آدنوما، آدنوئید سیستیک کارسینوما، پلی مورفوس low-grade آدنوکارسینوما، و موکواپیدرموئید کارسینوما ازجمله نئوپلاسم های شایع غدد بزاقی می باشند که با پیش آگهی های بسیار متفاوتی همراه می باشند. گاهی افتراق این نئوپلاسم ها از یکدیگر به دلیل همپوشانی های هیستوپاتولوژیک می تواند مشکل باشد.c-ki...
Commercial nucleic acid extraction kits are a cost effective, efficient and convenient way to isolate DNA and RNA from bacteria. Despite the increasing importance of the gastrointestinal pathogen, Clostridium difficile, and the increased use of nucleic acids in its identification, characterization, and investigation of virulence factors, no standardized or recommended methods for nucleic acid i...
Abstract Background and objectives: Paraffin-embedded tissues and clinical samples are a valuable resource for molecular genetic studies, but the extraction of high-quality genomic DNA from this tissues is still a problematic issue. In the Present study, the efficiency of two DNA extraction protocols, a commercial kit and a traditional method based on heating and K Proteinase was compared. Mate...
High quality DNA was isolated from Casuarina equisetifolia which is widely planted in tropics and subtropics for its multiple utilities. Two commonly used methods, CTAB based and the commercially supplied DNeasyTM Plant Mini Kit (QIAGEN GmbH, Germany) were tested for their efficiency in isolating high quantity and quality DNA to be utilized for AFLP analysis. The CTAB based protocol was modifie...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید