Leptospires are spirochetes that may be free-living saprophytes found in freshwater or may cause acute or chronic infection of animals. The family Leptospiraceae comprises three genera: Leptospira Leptospira Leptonema Leptonema, and Turneriella Turneriella. Within the genus Leptospira, three clades can be distinguished, of pathogens, nonpathogens, and an intermediate group. Leptospires are furt...

Serology plays an important role in laboratory diagnosis of leptospirosis. Apart from the most often used microscopic agglutination test (MAT), enzyme-linked immunosorbent assay (ELISA) seems to be useful especially in screenings of animal herds. The ELISA used for detection of antibodies against selected Leptospira serogroups in swine serum samples was investigated during the study. An essenti...

The prevalence of Leptospira interrogans serovars in dairy cattle was determined by analyzing 464 serum samples from cows on 15 properties in Garanhuns municipal district, Pernambuco State, Brazil. A microscopic seroagglutination test including 12 serovars of Leptospira interrogans as antigens was used. Samples with titres 100 were considered positive. Two hundred and twenty-one (47.63%) of the...

Comparative genomic hybridization was used to compare genetic diversity of five strains of Leptospira (Leptospira interrogans serovars Bratislava, Canicola, and Hebdomadis and Leptospira kirschneri serovars Cynopteri and Grippotyphosa). The array was designed based on two available sequenced Leptospira reference genomes, those of L. interrogans serovar Copenhageni and L. interrogans serovar Lai...

Leptospirosis caused by spirochete bacterium of genus Leptospira affects humans and animals worldwide. Rodents are major reservoirs of leptospires whereas wetland and aquatic migratory birds also carry and transmit leptospires. Leptospirosis studies in fish are lacking in African countries despite favourable environment and abundant reservoirs, which can spread leptospires into aquatic habitats...

Leptospirosis, a disease with protean manifestations, is caused by Leptospira species. Early and reliable identification of Leptospira serovars remains to be a challenge due to diversity at serovar level. The potential of polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) was assessed for detection of sequence variation in pathogenic specific 631 bp fragment of 16S r...

leptospirosis is an important infectious disease of animals and humans caused by the pathogenicleptospires which are classified into one species of leptospira interrogans containing over 212 serovars. thisstudy was conducted to determine the prevalence of leptospira-induced abortions in tabriz (north-west ofiran) dairy herds and to determine the pathogenic leptospira serovars responsible. from ...

BACKGROUND Long-term microscopic agglutination test (MAT) results after vaccination with 4-serovar Leptospira vaccines are not available for all vaccines used in client-owned dogs. HYPOTHESIS/OBJECTIVES To determine antibody responses of client-owned dogs given 1 of 4 commercially available Leptospira vaccines. ANIMALS Healthy client-owned dogs (n = 32) with no history of Leptospira vaccina...

The burden of leptospirosis in humans and animals in Africa is higher than that reported from other parts of the world. However, the disease is not routinely diagnosed in the continent. One of major factors limiting diagnosis is the poor availability of live isolates of locally circulating Leptospira serovars for inclusion in the antigen panel of the gold standard microscopic agglutination test...

In order to investigate the seroprevalence of leptospirosis in traditional dairy farms, a serological study was conducted in Gilan province. For this study,, a total of 205 serum samples were randomly collected from nine districts of this province. All serum samples were serologically tested by the standard MAT using live antigens representing the following Leptospira interrogans serovars: Grip...