RIDDICK, DANIEL H. Contractile vacuole in the amoeba, Pelomyxa carolinensis. Am. J. Physiol. 215(3): 736-740. 1968.-The osmolality of the cytoplasm and contractile vacuole of the giant amoeba Chaos chaos was determined by micropuncture. Sodium and potassium concentrations of the cytoplasm and contractile vacuole were similarly obtained. Single contractile vacuoles were isolated. It was determin...

Hydrangea macrophylla is popular for its unique physiological characteristics and changeable colors. Previous studies have shown that the pH of vacuoles sepal cells hydrangea affects color sepals. Located on vacuolar membrane, NHX1 an important H+ proton pump drives exchange metal ions. This environment by controlling accumulation in vacuole. In hydrangea, HmNHX1 gene has open reading frame 162...

Measurements of particle densities on the two freezefracture faces of digestive and perialgal vacuole mem­ branes in green and aposymbiotic Paramecium bursaria Ehrbg. show distinct differences between the P-faces of both membrane types. The distribution of particle den­ sities is more homogenous on the P-faces of perialgal vacuole membranes than on the P-faces of digestive vacuole membranes. Po...

The acidic food vacuole exerts several important functions during intraerythrocytic development of the human malarial parasite Plasmodium falciparum. Hemoglobin taken up from the host erythrocyte is degraded in the food vacuole, and the heme liberated during this process is crystallized to inert hemozoin. Several anti-malarial drugs target food vacuolar pathways, such as hemoglobin degradation ...

The intracellular life-cycle of Eimeria are located in the host cell within a membrane-bound parasitophorous vacuole. The invasion process and the formation of the parasitophorous vacuole are mediated by characteristic organelles within the apical complex. During invasion, the parasitophorous-vacuole membrane is manipulated by the parasite and functions later in the development cycle as a molec...

Vac8p is a multifunctional yeast protein involved in several distinct vacuolar events including vacuole inheritance, vacuole homotypic fusion, nucleus-vacuole junction formation and the cytoplasm to vacuole protein targeting pathway. Vac8p associates with the vacuole membrane via myristoylation and palmitoylation. Vac8p has three putative palmitoylation sites, at Cys 4, 5 and 7. Here, we show t...

Vacuole inheritance is temporally coordinated with the cell cycle and is restricted spatially to an axis between the maternal vacuole and the bud. The new bud vacuole is founded by a stream of vacuole-derived membranous vesicles and tubules which are transported from the mother cell into the bud to form the daughter organelle. We now report in vitro formation of vacuole-derived tubules and vesi...

In the budding yeast Saccharomyces cerevisiae, vacuoles are inherited by the projection of vesicles and tubules from the mother-cell vacuole into the growing daughter cell during the S phase. These vesicles then fuse and form the daughter-cell organelle. We have described previously in vitro reactions of the formation of vacuole-derived segregation structures and of vacuole-vacuole fusion. Homo...

The regulation of cellular membrane flux is poorly understood. Yeast respond to hypertonic stress by fragmentation of the normally large, low copy vacuole. We used this phenomenon as the basis for an in vivo screen to identify regulators of vacuole membrane dynamics. We report here that maintenance of the fragmented phenotype requires the vacuolar casein kinase I Yck3: when Yck3 is absent, salt...