This study is the first report of an infestation amoeba in gills asymptomatic Oreochromis sp. from Manir River, Terengganu, Malaysia. The results confirmed presence two species amoeba, Vermamoeba vermiformis and Tetratmitus sp., based on 18 ribosomal RNA gene sequences with 99% sequence similarity. Morphological observations using light scanning electron microscopy supported findings, demonstra...

Giant viruses continue to invade the world of virology, in gigantic genome sizes and various particles shapes. Strains discoveries and metagenomic studies make it possible to reveal the complexity of these microorganisms, their origins, ecosystems and putative roles. We isolated from a rat stool sample a new giant virus "Orpheovirus IHUMI-LCC2," using Vermamoeba vermiformis as host cell. In thi...

BACKGROUND The aim of this study was to compare between slow and rapid sand filters for the removal of free-living amoebae during drinking water treatment production. METHODS Overall, 48 water samples were collected from two drinking water treatment plants having two different filtration systems (slow and rapid sand filters) and from inlet and outlet of each plant. Water samples were collecte...

A real-time PCR-based method targeting the 18S rRNA gene was developed for the quantitative detection of Hartmannella vermiformis, a free-living amoeba which is a potential host for Legionella pneumophila in warm water systems and cooling towers. The detection specificity was validated using genomic DNA of the closely related amoeba Hartmannella abertawensis as a negative control and sequence a...

The isolation of giant viruses is of great interest in this new era of virology, especially since these giant viruses are related to protists. Giant viruses may be potentially pathogenic for many species of protists. They belong to the recently described order of Megavirales. The new lineage Faustovirus that has been isolated from sewage samples is distantly related to the mammalian pathogen Af...

Biodegradable compounds can cause undesired microbial growth in drinking water systems and these originate from the or pipe materials used systems. The aim of our study was to determine influence different on populations biofilm under semi-stagnant conditions. communities water, which were contact with seven materials, characterized by determining ATP concentrations, composition gene copy numbe...

Francisella tularensis is a gram negative facultative intracellular bacterium that causes the zoonotic disease tularemia. Free-living amebae, such as Acanthamoeba and Hartmannella, are environmental hosts of several intracellular pathogens. Epidemiology of F. tularensis in various parts of the world is associated with water-borne transmission, which includes mosquitoes and amebae as the potenti...