objective: this study was carried out to examine the effect of vero cells co-culture on developmental competence of immature oocytes. materials and methods: bovine cumulus-oocyte-complexes (cocs) were matured in either presence or absence of vero cells. matured oocytes were inseminated and cultured for up to nine days. cleavage percentages were recorded on day two post insemination (pi). embryo...

introduction: the purpose of this study was to investigate the effect of rpmi and t6 medium on two cell mouse embryo co-culture with vero cells. materials and methods: two cell mouse embryos (nmri strain) were flushed from the excised uteri of superovulated mice. morphologically normal embryos were divided into four groups: the first control group was cultured on t6 media. the first experimenta...

UNLABELLED A hepatitis C virus (HCV) cell culture system incorporating the JFH-1 strain and the human hepatoma cell line HuH-7 enabled the production of infectious HCV particles. Several host factors were identified as essential for HCV replication. Supplementation of these factors in nonhepatic human cell lines enabled HCV replication and particle production. Vero cells established from monkey...

Background: Morphological changes of the cells infected with rubella virus cannot be observed easily. Estimation of the size of the cultured cells can be a valuable parameter in this condition. This study was conducted to find answers to the following questions:How much time after infection with rubella virus, the volume and surface area of the Vero cells and their nuclei get started to change?...

Diphtheria toxin has the capacity to block protein synthesis in cultured mammalian cells, and thus causing cell death. This capacity of diphtheria toxin was utilized for in-vitro neutralization test to determine antibody titer, using Vero cells, which have been found to be susceptible to diphtheria toxin. In the present study, a Vero cell assay was standardized and validated for potency estimat...

DTS-II is a highly diphtheria toxin (DT)-sensitive cell line previously isolated by transfection of wild-type DT-resistant mouse L-M(TK-) cells with the cDNA encoding a monkey Vero cell DT receptor. DTS-II cells are as toxin-sensitive as Vero cells, have approximately 3-fold more receptors than Vero cells, and have approximately 10-fold lower affinity for DT than Vero cells. We now cotransfecte...

background: morphological changes of the cells infected with rubella virus cannot be observed easily. estimation of the size of the cultured cells can be a valuable parameter in this condition. this study was conducted to find answers to the following questions: how much time after infection with rubella virus, the volume and surface area of the vero cells and their nuclei get started to change...

Vero cells used by distinct measles vaccine control laboratories had their susceptibility to Moraten, Schwarz and Biken CAM-70 vaccine strains assayed. Of a total of 72 lots of measles vaccine whose potency was titrated by microtechnique in two Vero cell samples (Vero IB and Vero INCQS), 25 had been produced with Moraten strain, 24 with Schwarz and 23 with Biken CAM-70. The statistical analysis...

introduction: this study was started to improve in vitro blastocyst development and avoiding of blastocyst collapsment (no visibie cavity, returning of hatching blastocyst into zona pellucida, and tendency to degeneration). materials and methods: 24hrs one - cell mouse embryos were recovered from positive plug females and cultured in following canditions: experiments 1,2: during first 48hrs nmr...