The study of alveolar macrophages (AM) from smokers by flow cytometry (FCM) has been limited by strong autofluorescence and the lack of reliable markers to identify macrophages. Crystal violet quenching was reported to be effective in reducing autofluorescence of AM. CD68 is a marker for macrophages in immunohistochemistry, but has been less useful in FCM because of poor surface expression. Thi...

Whereas humans have three types of cone photoreceptor, birds have four types of single cones and, unlike humans, are sensitive to ultraviolet light (UV, 320-400 nm). Most birds are thought to have either a violet-sensitive single cone that has some sensitivity to UV wavelengths (for example, many non-passerine species) or a single cone that has maximum sensitivity to UV (for example, oscine pas...

We demonstrate a new type of interaction between suprathreshold colour (chromatic) and luminance contrast in the context of binocular, specifically dichoptic vision. A highly saturated isoluminant violet 'mask' disk in one eye greatly elevates detection thresholds for an isoluminant violet 'test' disk in the other eye, an example of dichoptic colour-saturation masking. However when binocular lu...

Fungi is widely found on air, soil, water and organic substances in nature. Unlike single-celled bacteria, fungi form multicellular structures, sometimes even visible to the naked eye. In studies of Aspergillus sp. removal dye order obtain information, examinations are made by painting. Basic dyes, when ionized, charged with positive electricity. dyes include malachite green, methyl violet, cry...

1. It is found that with many indicators there is a big discrepancy between the true alkalinities or pH values of solutions of pure sodium hydroxide and of standard alkaline buffers which give the same actual colour. This discrepancy must be ascribed to salt error caused by the buffer itself and exceeds in the most extreme case two whole units of pH; that is, an error of 100 fold in determining...