A new method of measuring the growth of Lactobacillus leichmannii is reported. Its adoption for the estimation of serum vitamin B(12) levels shortens the incubation period required to five hours at 45 degrees C. The method is compared statistically with a standard method of estimation, requiring incubation at 37 degrees C., by duplicate determinations on 106 hospital patients. The significance ...

The final stages in the total synthesis of vitamin B12 are reported. It was particularly appropriate, I think, and most pleasant for us this morning to see the splendid film provided by our Russian friends, in which the very successful Symposium at Riga was enshrined for posterity. Appropriate in the sense, from my own point of view, that I did report there at Riga, on the progress we had made ...

as we know, no attempts to compare quantitatively the expected amount prescribed, on the basis of good clinical practice, with that actually prescribed. There are in general three reasons for this-ignorance of the prevalence of common diseases, the lack of specificity of particular drugs for particular diseases, and the lack of standardization of therapy. Such a study requires a drug which is p...

INTRODUCTION In 19'58 Barker and his associates l 3 recognized a new coenzyme which controlled the conversion of glutamate into ,8-methylaspartate by Clostridium tetanomorph'im. The coenzyme was shown4 to be related to ",-vitamin B12, i.e. contair ing an adenine nucleotide grouping in place of the 5,6-dimethylbe:nziminalole nucleotide of vitamin B12, although similar coenzymes containing b{ nzi...

The present study was performed to investigate the effects of long-term intraperitoneal (IP) injection of vitamin B12 and diclofenac in separate and combined treatments on cold and mechanical allodynia in a neuropathic pain model in rats. Neuropathic pain was induced by crush injury in right tibial nerve. Acetone spray and von Frey tests were used to obtain cold and mechanical allody...