BACKGROUND The role of wild birds in the transmission and spread of mycoplasmas is not clear. Up to now different Mycoplasma species have been isolated from wild birds many of which are not considered pathogens sensu stricto for domestic flocks. This report describes the first isolation of Mycoplasma synoviae in a captive lesser flamingo (Phoeniconaias minor) held in a zoo in Italy and the labo...

Detection of Mycoplasma synoviae (MS) by culture and polymerase chain reaction (PCR) has been reported from commercial chicken farms in different provinces of Iran. In some reports the phylogenetic analysis of MS isolates based on 16S rRNA and variable lipoprotein hemagglutinin (vlhA) genes have been carried out. The PCR product containing partial 16S rRNA genes of Iranain isolates was sequence...

Temperature-sensitive (ts+) vaccine strain MS-H is the only live attenuated M. synoviae vaccine commercially available for use in poultry. With increasing use of this vaccine to control M. synoviae infections, differentiation of MS-H from field M. synoviae strains and from rarely occurring non-temperature-sensitive (ts-) MS-H revertants has become important, especially in countries where local ...

Mycoplasma 352 Molecular characterization of mycoplasma gallisepticum isolated from chicken and turkey. 353 Pathogenicity of Mycoplasma gallisepticum strains using ELD50 in embryonated chicken eggs. 354 Evaluation of three DNA extraction methods for the detection of Mycoplasma spp. with an MG/MS multiplex real-time PCR method. 356 Use of MG/MS serology and PCR to determine flock status. 357 Exp...

Avian mycoplasmas are associated with respiratory disease, synovitis, poor quality of day-old chicks and performance. The main approach used for the diagnosis avian is isolation identification microorganism. Since bacteria slow growing fastidious organisms, conventional methods time consuming, laborious require experienced personnel. For this reason, we aimed to develop a rapid detection method...