The effect of seminal plasma, as well as some seminal plasma fractions, on the heterogeneity and viability of frozen-thawed ram spermatozoa was studied. Fresh and frozen sperm samples were simultaneously assessed by a routine semen-quality assay and centrifugal countercurrent distribution (CCCD) in an aqueous two-phase system analysis. All samples used for this study were obtained from Salz ram...

The aim of this study was to cryopreserve and evaluate frozen-thawed semen parameters. Electroejaculator was used to collect semen from six bucks. Semen samples were divided into two parts. In one part, seminal plasma was removed and was not in the other one. Both semen sample parts were extended with egg-yolk Tris extender. Semen was thereafter cryopreserved in straws using liquid nitrogen. Da...

Neisseria gonorrhoeae of pilated T1 and nonpilated T4 colony types attached themselves to human sperm in greatest numbers and most reproducibly when suspensions of the cells were incubated at 35 degrees C in Ringer's solution, pH 6-8. After incubation for 15 or 30 min. in a water bath shaker, 50 per cent. of human sperm had T1 gonococci attached and 25 per cent. T4. Sperm and both types of gono...

The active interest in determining the ultraThe washed sperm samples were then treated with structural organization of ejaculated human sperm alkaline thioglycolate (18) . A 0 .8 M concentration of nucleus by the normal thin-sectioning technique alkaline thioglycolate was prepared by raising the has led to a state of understanding that is still pH to 9 with I N NaOH. This reagent was mixed inco...

background: ubiquitin, an 8.5 kda peptide that marks other proteins for proteasomal degradation, tags defective sperm dur­ing epididymal passage. thus, sperm ubiquitination is a universal marker for sperm defects and can be used as a sperm func­tion test. the objective of the present study was to examine the relationships between sperm ubiquiti­nation and clinical se­men parameters, using simpl...

CD52 is a glycosylphosphatidylinositol-anchored glycoprotein secreted by the epididymis where it is incorporated into sperm membranes. The antigen is common to spermatozoa and lymphocytes, and its role is not known. Quantitative analysis using immunostaining with the monoclonal antibody CAMPATH-1G and flow cytometry indicated positive signals from approximately 80% of viable, washed ejaculated ...

Ejaculated, bovine sperm have been subjected to multiple partition in aqueous two-phase systems. This partition, carried out in a countercurrent fashion, reveals heterogeneity of the sperm population with respect to surface properties. The sperm, when partitioned in phase systems that detect non-change associated surface properties (change-insensitive) are largely distributed as two distinct po...

The primary goal of this work was to test whether the sperm mobility assay could be used to derive mathematical relationships from which predictions could be made about sperm cell function. A precondition was random sampling from a pool of sperm. This precondition was met by centrifuging mobile sperm through 12% (wt/vol) Accudenz containing the Ca(2+) chelator 1,2-bis-(o-aminophenoxy)ethane-N,N...