background: watermelon silver mottle virus (wsmov), which belongs to the genus tospovirus, causes significant loss in cucurbitaceae plants. objectives: development of a highly sensitive and reliable detection method for wsmov.materials and methods: recombinant plasmids for targeting the sequence of nucleocapsid protein gene of wsmov were constructed. sybr green i real-time pcr was established a...

In North Carolina, approximately 8% of the cucumber (Cucumis sativus) yield is lost to root-knot nematodes (Meloidogyne spp.) (Main and Gurtz, 1989). No resistance was found in cucumber after screening of 900 cultigens for resistance to Meloidogyne incognita race 3 (Walters, 1991). African horned cucumber (Cucumis metuliferus) has resistance to M. incognita (Fassuliotis, 1967; Walters, 1991), a...

Recent studies have documented effects of plant viruses on host plants that appear to enhance transmission by insect vectors. But, almost no empirical work has explored the implications of such apparent manipulation for interactions among co-infecting pathogens. We examined single and mixed infections of two potyviruses, watermelon mosaic virus (WMV) and zucchini yellow mosaic virus (ZYMV), tha...

Transmission experiments of different isolates of Cucumber mosaic virus (CMV), Watermelon mosaic virus (WMV) and Zucchini yellow mosaic virus (ZYMV) were conducted using established colonies of Aphis gossypii Glover, Myzus persicae (Sulzer), A. fabae Scopoli and A. craccivora Koch as vectors. The transmission procedure used provided an estimate of the virus transmission efficiency for each aphi...