Western blotting is a commonly used technique in biological research. A major problem with Western blotting is not the method itself, but the use of poor quality antibodies as well as the use of different experimental conditions that affect the linearity and sensitivity of the Western blot. Investigation of some conditions that are commonly used and often modified in Western blotting, as well a...

in this study 19 blv infected and 2 healthy lymph nodes were analysed by western blotting to detect viral p 24 antigen . western blotting was carried out by rabbit anti p24 serum and p24 monoclonal antibody. rabbit serum in western blotting could detect 45, 50, 54, 58 and less than 19 kd bands of proteins and monoclonal antibody could detect 30, 32, 39, 45, 50 and 53 kd bands. our results indic...

cholera toxin b subunit (ctb) has been extensively studied as an immunogen, adjuvant, and inducer of oral tolerance in many investigations. production of ctb has been carried out in the bacterial, plant, insect and yeast expression systems. in this study the expression of the ctb containing a 6xhis-tagged was performed by escherichia coli (e.coli) m15. the yield of purified pentameric recombina...

background and objective: neisseria meningitidis is a leading cause of meningitis and sepsis worldwide. the factor h binding protein (fhbp) is a key virulence factor of neisseria meningitidis that is able to selectively bind to human factor h, the key regulator of the alternative complement pathway, which it has important implications for meningococcal pathogene- sis and vaccine design. the aim...

background: human cd34 is a transmembrane glycoprotein which is expressed in human hematopoietic stem cells (hscs) and the small- vessel endothelial cells of a variety of tissues. cd34 plays a critical role as a marker for diagnosis and classification of leukemia. anti cd34 antibodies are used for isolation and purification of hscs from bone marrow, peripheral blood and cord blood. objective: t...