The Bactec 9240 and the BacT/Alert blood culture systems were compared as a means for detection of bacterial contaminants in whole blood, concentrated red cells, and plasma preparations prepared from umbilical cord blood (UCB) samples. Ninety-two UCB units seeded with low levels of various bacteria were evaluated. In more than 50% of cases, growth was not detected in plasma using either system ...

BACKGROUND Sepsis is a serious medical condition requiring timely administered, appropriate antibiotic therapy. Blood culture is regarded as the gold standard for aetiological diagnosis of sepsis, but it suffers from low sensitivity and long turnaround time. Thus, nucleic acid amplification tests (NAATs) have emerged to shorten the time to identification of causative microbes. The aim of the pr...

Background: The metaphase-spread-based dicentric chromosome assay (DCA) is the “gold standard” biodosimetry method for radiation dose assessment. However, practical issues influencing dose assessment by DCA, such as cell culture type and blood transport temperature are not addressed. Purpose: To fill the research gaps, we studied the effects of short-term cell culture type, the use of whole blo...

BACKGROUND Rapid detection of bloodstream infections (BSIs) can be lifesaving. We investigated the sample processing and assay parameters necessary for highly-sensitive detection of bloodstream bacteria, using Staphylococcus aureus as a model pathogen and an automated fluidic sample processing-polymerase chain reaction (PCR) platform as a model diagnostic system. METHODOLOGY/PRINCIPAL FINDING...

A number of modifications of Moorhead's original technique for culturing peripheral leucocytes have been described, enabling cultures to be grown from a few drops of blood (Arakaki and Sparkes, I963; Edwards, I962; Robinson, Bishun, Rashad, and Morton, I964). They require the use of stored calf, chick, or human serum, and this has obvious disadvantages for the small laboratory. We have, therefo...

conclusions the etiology of exchange transfusion was unidentified in most cases; however, abo incompatibility was the most prevalent cause of hyperbilirubinemia. complications were common after exchange transfusion and should be considered carefully. patients and methods a retrospective study was performed on 28 days old infants who received exchange transfusion due to severe hyperbilirubinemia...