A laboratory study comparing the Widal slide agglutination test using local antigens produced by Mekar Jaya Diagnostica (SAT-MJD) with imported antigens (Murex, Abbott) was carried out on 55 sera of typhoid fever patients with positive blood culture and 56 sera of non-typhoid febrile patients. The SAT-MJD antigens consisted of a mixture of 5 different phage-types of S. typhi dominantly found in...

Typhoid fever remains a major public health risk with more than 13 million cases occurring annually in Asia alone. Delay in diagnosis and institution of appropriate therapy especially with emergence of multi-drug resistant strains of Salmonella typhi, can be associated with significant morbidity and mortality may significantly increase the risk of adverse outcome. Traditionally, the isolation o...

Enteric fever is a systemic infection caused by Salmonella serotypes; S. typhi, S. paratyphi A, S. paratyphi B, S. paratyphi Cand S. sendai (Mandell GL et al, Parker MT). It is characterized by malaise, fever, abdominal discomfort, transient rash, splenomegaly, hepatomegaly, bradycardia, and leucopenia. Major complications are intestinal hemorrhage and perforation (Hook EW et al). It is still a...

Enteric fever is endemic in this part of the world, and Widal test is one of the time-honored laboratory tests that are being used for years to diagnose the disease. On the other hand, melioidosis is a newly emerging disease from this region, which is most often misdiagnosed or underdiagnosed by clinicians. It is well accepted that false-positive Widal reactions following certain non-typhoid Sa...

The sensitivity and specificity of counterimmunoelectrophoresis using three antigenic preparations obtained from Salmonella typhi were compared with those of the Widal test in the serological diagnosis of typhoid fever. A veronal buffer extract yielded precipitation lines against 50 (96%) out of 52 sera collected from patients with typhoid but against none out of 62 sera obtained from control s...

Reliability of the Widal tube agglutination test has been the subject of many controversies over the years. This study was performed to assess the effect of certain modifications on the performance of Widal test in a novel microplate assay. Sera from 37 patients (21 males; 16 females) (mean age 28 +/- 7 years) were tested in the Immunology Unit at King Khalid University Hospital, Riyadh. Among ...

We review the significance of the Widal agglutination test in the diagnosis of typhoid fever. Over 100 years since its introduction as a serologic means of detecting the presence of typhoid fever, the Widal test continues to be plagued with controversies involving the quality of the antigens used and interpretation of the result, particularly in endemic areas. Areas of concern with clinical and...

BACKGROUND Enteric fever is endemic in all parts of India. The Widal agglutination test is widely used for its diagnosis. But the interpretation of Widal test depends upon the baseline titre which is prevalent amongst healthy individuals in a particular geographical area. AIM Present study was undertaken to establish the normal baseline titre in apparently healthy blood donors and also to det...

Reliability of the Widal tube agglutination test has been the subject of many controversies over the years. !is study was performed to assess the e$ect of certain modifications on the performance of Widal test in a novel microplate assay. Sera from 37 patients (21 males; 16 females) (mean age 28 + 7 years) were tested in the Immunology Unit at King Khalid University Hospital, Riyadh. Among them...