The protamine mRNAs are stored for up to 8 days as translationally repressed ribonucleoprotein particles during murine spermatogenesis. Translational repression of the protamine 1, Prm1, mRNA is controlled by sequences in its 3'-untranslated region (UTR). In this study we used the yeast three-hybrid system to clone Msy4, which encodes a novel member of the Y box family of nucleic acid binding p...

R NA binding proteins have long been known to play a role in spermatogenesis. The laboratory of Norman Hecht was one of the leaders in this field, having characterized three RNA binding proteins that regulate mRNAs during sperm differentiation. Polypyrimidine tract-binding protein 2 (PTB2) has two functions—stabilizing mRNA transcripts in the cytoplasm and being involved in splicing. Using in v...

Cystic fibrosis is the most common genetic disease among Caucasians and affects tissues including lung, pancreas and reproductive tracts. It has been shown that Endoplasmic Reticulum (ER) stress and heat shock response are two major deregulated functional modules related to CFTR dysfunction. To identify the impact of CFTR deletion during spermatogenesis, we examined the expression of spermiogen...

L-Cvsteine-S-conjugates are responsible for the mutagenic and c\ totoxic consequences which occur following exposure to certain xenobioticsl I I Following enLyme mediated C-S lysis of the cc steine conjugate. equimolar amounts of pyruvate, ammonia and a thiolate species are formed (Fig 1 ) It has now been established that one of the physiological rdles of C-S lyase enzymes is that of transamina...

The oocyte is a remarkable haploid cell that carries all the information needed to undergo a complex maturation process to become a fertilizable egg and possesses the ability to initiate the early stages of development after fertilization. During the period of oocyte growth, mRNAs are synthesized and stored, and these mRNAs are very stable at this time, with a half-life of approximately 12 days...

STUDY QUESTION Is the postovulatory aging-dependent differential decrease of mRNAs and polyadenylation of mRNAs coded by maternal effect genes associated with altered abundance and distribution of maternal effect and RNA-binding proteins (MSY2)? SUMMARY ANSWER Postovulatory aging results in differential reduction in abundance of maternal effect proteins, loss of RNA-binding proteins from spec...