plant fatty acyl-acyl carrier protein thioesterase (fat) is a major enzyme regulating the amount and composition of fatty acids in lipids. in this study, one type of cdna, corresponding to the fatty acyl-acyl carrier protein (acyl-acp) thioesterase (fat) enzyme, was isolated from the seed of brassica napus cv. ningyou12. blast results revealed that the cdna identified highly with the fatb class...

background: toxoplasma gondii is an obligate intracellular parasite and its sexual and asexual cycles, respectively take place in the intestinal epithelial cell of definitive host and tissue of intermediate hosts. congenital toxoplasmosis is more impor tant when the mother acquired the infection during pregnancy period for the first time. serological tests are the only meth ods for diagnosis of...

We have designed and constructed a novel expression vector capable of producing recombinant proteins in both mammalian cell lines and the yeast strain Pichia pastoris. In this vector, a yeast promoter is placed inside an intron of the mammalian transcription unit. A yeast transcription termination sequence is placed immediately downstream of the mammalian polyadenylation site. In mammalian cell...

infectious bursal disease virus (ibdv) is the causative agent of gumboro disease, an infectious disease of global economic importance in poultry. the expression of heterologous proteins in p.pastoris is fast, simple and inexpensive. in this study, vp2 encoding gene of classical d78 ibdv was amplified using reverse transcription (rt) polymerase chain reaction (pcr) and cloned into ppiczαa vector...

Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. The expression of heterologous proteins in P.pastoris is fast, simple and inexpensive. In this study, VP2 encoding gene of classical D78 IBDV was amplified using reverse transcription (RT) polymerase chain reaction (PCR) and cloned into pPICZαA vector...

Background: Equine follicle stimulating hormone (eFSH) is a pituitary heterodimeric glycoprotein consists of noncovalently linked of generic alpha subunit and a hormone specific beta subunit. The molecular weights of the subunits are similar and about 16 KD. In general, FSH plays a key role in controlling vertebrate gonadal functions. In female mammals, ovarian maturation and follicular growth ...

conclusions our results showed that the recombinant protein produced by the pbad vector in the escherichia coli system was very efficient. background nontypeable haemophilus influenzae (nthi) is a significant pathogen in children, causing otitis media, sinusitis, conjunctivitis, pneumonia, and occasionally invasive infections. protein d (pd) belongs to the minor outer-membrane proteins of h. in...

Background & Aims: Vibrio cholerae is a gram-negative bacterial pathogen that causes cholera disease. Following ingestion by a host and entry into the upper intestine, V. cholera colonizes and begins to emit enterotoxin. One of the most pathogenic factors of Vibrio cholera is toxin-coregulated pili (TCP). ToxinCoregulated pili is as the primary factor requiered for the colonization and insisten...

SETH4 coding sequence with 2013 bp is a member of gene family expressed in gametophytic tissues of Arabidopsis thaliana. This fragment was PCR amplified using Kod Hi Fi DNA polymerase enzyme. This fragment was cloned into pGBKT7 bate vector and transformed E. coli DH5? cells containing vector were selected on LB medium containing Kanamycin. Finally, pGBKT7-SETH4 bate was transformed into yeast ...

Heterologous protein production in yeast expression systems (i.e., Kluyveromyces lactis and Pichia pastoris) normally involves insertion of a linear expression cassette into a target locus in the host genome (1-3). Typically, an expression cassette is assembled in E. coli by first cloning a gene of interest into a circular expression vector (Figure 1A). The expression cassette comprises DNA enc...