androstane skeleton with a keto-enol tautomerism at the cyclopentane ring. Wecarried out further isolation for PFTase inhibitors from the cultured broth of strain FO-3929 and found andrastin D (4, Fig. 1). In this paper, fermentation, isolation, structure elucidation, and biological properties of 4 are described. A stock culture of strain FO-3929 was inoculated into six 500-ml Erlenmeyer flasks...

several strains of saccharomyces cerevisiae were isolated from different sources. these strains were under taken for ethanol tolerance analysis and growth under stress condition. exponential phase to various concentrations of ethanol (2–26% v/v) for 1 h was used for isolation of resistant strains. viable cells were isolated and purified by inoculation of diluted samples on yeast extract peptone...

The aim of the study was to evaluate the accuracy of CHROMagar-Candida for the identification of Candida spp. from different clinical specimens. The growth of cultures on plates, containing Sabouraud dextrose agar and CHROMagar-Candida were compared. Non-Candida albicans yeast isolates were further specificated with API 20C (bioMerieux, France). Of the 178 cultures processed, 100% accuracy for ...

normal 0 false false false en-us x-none ar-sa background: invasive fungal infections cause considerable morbidity and mortality in immunocompromised hosts. pigeon droppings could especially be a potential carrier in the spread of pathogenic yeasts and mold fungi into the environment. the objective of this study was to isolation of cryptococcus neoformans and other opportunistic fungi from pigeo...

The present investigation is primarily concerned with the isolation of p-aminobenzoic acid from yeast. The presence of this substance in yeast was adumbrated by Woods (1) who made the important discovery that. this aromatic amino acid possesses the unique propcity of antagonizing the bsctcriostat~ic action of sulfanilamidt? and that the component. of yeast extracts which exerts a similar antisu...

INTRODUCTION The aim of this study was to create a predictive score for yeast isolation in patients with complicated non-postoperative intra-abdominal infections (CNPIAI) and to evaluate the impact of yeast isolation on outcome. METHODS All patients with a CNPIAI undergoing emergency surgery over a three-year period were included in the retrospective cohort (RC, n = 290). Patients with a yeas...

Knowledge of the mechanisms that lead to reproductive isolation is essential for understanding population structure and speciation. While several models have been advanced to explain post-mating reproductive isolation, experimental data supporting most are indirect. Laboratory investigations of this phenomenon are typically carried out under benign conditions, which result in low rates of genet...

We describe a technique that facilitates the isolation of yeast genes that are difficult to clone. This technique utilizes a plasmid vector that rescues lambda clones as yeast centromere plasmids. The source of these lambda clones is a set of clones whose location in the yeast genome has been determined by L. Riles et al. in 1993. The Escherichia coli-yeast shuttle plasmid carries URA3, ARS4 an...

There is a need for new approaches to isolate thermotolerant yeast strains that can be utilized for the efficient production of ethanol. The simultaneous saccharification and fermentation of starch or lignocellulosic will greatly benefit from thermotolerant yeast strains that actively ferment ethanol at temperatures above 40°C. The development of new procedures targeting the cell membrane to in...