OBJECTIVE To evaluate the performance of GeneXpert for detection of mycobacterium tuberculosis in pus samples and compare its results with conventional techniques in terms of validity, rapidity and rifampicin resistance. METHODS This longitudinal, descriptive study was conducted at Jinnah Hospital, Lahore, Pakistan, from January 2012 to December 2015, and comprised pus samples of people suspe...

faecal samples from 291 dairy calves from 10 dairy herds in kerman were examined to investigate theprevalence of cryptosporidium infection. cryptosporidium spp. oocysts were identified by using themodified ziehl-neelsen staining technique in 63 (21.65%) calves. calves were grouped according to theirage as follows:

We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in...

If tubercle bacilli could be easily detected in clinical samples with gram staining, it would be possible to detect tuberculosis more promptly and easily, which may contribute to tuberculosis control. In this paper, we present three infective tuberculosis cases in which gram staining easily detected tubercle bacilli before Ziehl-Neelsen (Z-N) staining and diagnosed by polymerase chain reaction ...

The purpose of our survey is to estimate the frequency Cryptosporidium spp in dairy calves under 60 days age, pathogenic role development diarrhea, intervention and association other enteropathogens often involved neonatal diarrhea possible relationship between infection certain farming practices order share risk factors, on hand, compare modified Ziehl-Neelsen staining method copro-ELISA test....

Objective. To determine the Cryptosporidium spp. infection frequency by using Ziehl-Neelsen and Auramine stains on samples obtained from diarrheic calves from milking farms of the Valdivia province. To compare both diagnostic tests and to determine the geospatial distribution of the infections caused by this protozoan. Materials and methods. 221 fecal samples of diarrheic calves of 24 milking f...