1Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA; 2Department of Animal Science, University of Minnesota, Saint Paul, Minnesota, USA; 3Department of Genetics, Cell Biology and Development, Minneapolis, Minnesota, USA Correspondence: Perry B Hackett, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, US...

Genome editing is an exciting technology that allows for specific manipulation of complex genomes. While the original tools for genome manipulation had low efficiencies, genome editing tools discovered in the past fifteen years have been widely studied and great efforts have been required to improve their efficiency. This article summarizes how Zinc Finger Nuclease (ZFN), Transcription Activato...

Glioblastoma is a malignant brain cancer with high mortality. Currently, there no effective treatment that can effectively improve the survival of patients. Traditional gene editing tools, such as Zinc finger nuclease, RNAi and translational activator-like effector nucleases (TALENTS), have shown their advantages in treating glioblastoma. However, they disadvantages cost difficult to operate. I...