A thermostable and detergent-stable α-amylase from a newly isolated Anoxybacillus sp. AH1 was purified and characterized. Maximum enzyme production (1874.8 U/mL) was obtained at 24 h of incubation. The amylase was purified by using Sephadex G-75 gel filtration, after which an 18-fold increase in specific activity and a yield of 9% were achieved. The molecular mass of the purified enzyme was est...

Effect of Seaweed Liquid Extract (SLE) of Caulerpa scalpelliformis on growth and biochemical constituents of Vigna mungo was studied. The lower concentration of SLE of C. scalpelliformis (25%) enhanced the percentage of germination, shoot length, root length and biochemical constituents viz., chlorophyll, carotenoid, amino acid, reducing sugar and total sugar contents and α-amylase and β-amylas...

Waste oil industry has a great potential use, as 77% oil yield are an untapped waste that still has a high economic value as a base for the oleochemical industry, one of which is β-carotene. The study was conducted to optimize the extraction of carotenoids from Crude Palm Oil (CPO) and Palm Oil Mill Effluent waste (POME) conducted by column chromatography and extraction method with a single enz...

Rap1 has recently been identified on the secretory granule membrane and plasma membrane of rat parotid acinar cells (N. J. D'Silva, D. DiJulio, C. B. Belton, K. L. Jacobson, and E. L. Watson. J. Histochem. Cytochem. 45: 965-973, 1997). In the present study, we examined the cellular redistribution of Rap1 following treatment of acini with isoproterenol (ISO), the β-adrenergic agonist, and determ...

This study was designed to determine the effect of exogenous spermidine (Spd) (30 μM) on white clover seed germination under water stress induced by polyethylene glycol 6000. Use of seed priming with Spd improved seed germination percentage, germination vigor, germination index, root viability and length, and shortened mean germination time under different water stress conditions. Seedling fres...

Amount of chlorophyll content, Proteins, Carbohydrates and RNA has been studied in ten taxa of Mosses. The study also embodies the specific activity of enzymes α-amylase, ß-amylase, Proteases and polyphenol oxidases. It has been found that carbohydrate content is more than protein content in Atrichum pallidum, Thuidium recognitum, Brachythecium kamounense and Funaria hygrometrica, whereas, tota...