background: the aim of this study was to clone pparγ1 cdna in an appropriate mammalian expression vector, with a chimeric cdna form, encompassing pparγ with enhanced green fluorescent protein (egfp) cdna. this recombinant plasmid will be used for further analyses to investigate the molecular mechanism of pparγ1 for neural differentiation process. moreover, the nuclear localization of the pparγ1...

Suppression subtractive hybridization (SSH) is a widely used method for separating DNA molecules that distinguish two closely related DNA samples. Two of the main SSH applications are cDNA subtraction and genomic DNA subtraction. In fact, SSH is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. The SSH method is based on a suppression PCR effe...

tree nuts, including almond (prunus dulcis) are a source of food allergens often associated with life-threatening allergic reactions in susceptible individuals. although the proteins in almonds have been biochemically characterized, relatively little has been reported regarding the identity of the allergens involved in almond sensitivity. the present study was undertaken to identify the allerge...

امروزه ورود به حوزه مدیریت کیفیت برای سازمان‌ها از اهمیت ویژه‌­ای برخوردار است. مدل­های تعالی سازمانی ابزاری است که را در این مسیر رهنمون می­‌کند. بدین منظور، ازجمله شبکه بانکی کشور، رویکردهای گوناگونی سازمان خود راستای تحقق ارزش‌های مدل­‌های استقرار می­کنند؛ اما محدودیت منابع موجود قبیل هزینه، نیروی انسانی، فرهنگ و غیره، تمامی رویکردها میسر نمی‌­سازد؛ ازاین‌رو مسئله اصلی کدام بیشترین تأثیر ارت...

A label-free colorimetric method based on exonuclease I (Exo I)-assisted signal amplification with protamine as a medium was developed for analysis of kanamycin. In this study, double-stranded DNA (dsDNA) probe tailored by manipulating an aptamer and its complementary (cDNA) ensuring detection target high selectivity excellent sensitivity. Herein, could not only combine negatively charged gold ...