نتایج جستجو برای: دورگهسازی dna ژنومی در محل gish
تعداد نتایج: 1257756 فیلتر نتایج به سال:
Four accessions of hexaploid Elymus repens from its native Central European distribution area were analyzed using sequencing of multicopy (internal transcribed spacer, ITS) and single-copy (granule-bound starch synthase I, GBSSI) DNA in concert with genomic and fluorescent in situ hybridization (GISH and FISH) to disentangle its allopolyploid origin. Despite extensive ITS homogenization, nrDNA ...
to remove unfavorable traits of new cereal tritipyrum (2n=6x=42, aabbebeb), various crosses between primary tritipyrum lines (female) with iranian bread wheat varieties (2n=6x=42, aabbdd) was performed and a f2 selfing population consisted of 1810 possible secondary tritipyrum seeds (2n=6x=42, aabbd(0-14) eb(0-14)) were produced. the chromosome constitution and homology index of 11 plants of th...
BACKGROUND AND AIMS Repetitive DNA sequences are thought to be involved in the formation of chromosomal rearrangements. The aim of this study was to analyse the distribution of microsatellite clusters in Aegilops biuncialis and Aegilops geniculata, and its relationship with the intergenomic translocations in these allotetraploid species, wild genetic resources for wheat improvement. METHODS T...
Background: Non-Iranian Primary Tritipyrum (2n=6x=42, AABBEbEb) set seed after Triticale (2n=6x=42, AABBRR) and Tritordeum (2n=6x=42, AABBHcHc) but, due to a few undesirable agronomic traits, it cannot fulfil the commercial expectations of farming. Objectives: To remove these deficiencies, six hexaploid Tritipyrum lines were crossed with four Iranian bread wheat cultivars which led to the...
Top, tree or Egyptian onion, Allium x proliferum (Moench) Schrader, 2n=2x=16, is a minor, vegetatively propagated garden crop in Europe, North America and North East Asia. Several clones of the top onion were found to be locally cultivated in the region of South Croatia under the name “Ljutika-talijanka”. One of these, clone Biorina, was studied by means of both classi cal cytogenetic techniqu...
BACKGROUND The intergeneric hybrids between Ascocenda John De Biase 'Blue' and Phalaenopsis Chih Shang's Stripes have been generated to introduce the blue color into the Phalaenopsis germplasm in prior study. In order to confirm the inheritance in hybrid progenies, genomic in situ hybridization (GISH) and restriction fragment length polymorphism (RFLP) analysis were conducted to confirm the int...
چکیده ندارد.
استخراج dna بهصورت خالص، پایدار و با کمیت و کیفیت مناسب، گام مهمی در اجرای پژوهشهای بیولوژیکی میباشد. در این پژوهش، dna مربوط به نمونه خون 600 بلدرچین ژاپنی با روش نمکی تغییر یافته با پودر رختشویی و بدون استفاده از آنزیم پروتئیناز k استخراج شد. نمونههای dna بهدست آمده در مراحل آمادهسازی کتابخانه ژنومی، با نانودراپ، فلئورومتر، بیوآنالیزر، الکتروفورز، pcr، هضم با آنزیمهای آندونوکلئاز، اتصا...
Genomic in situ hybridization (GISH) has been widely used to detect rye (Secale cereale L.) chromosomes in wheat (Triticum aestivum L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeling of genomic DNA of rye and denaturing of chromosomes and probes. In this study, new oligonucleoti...
مکانیزم شکست ناشی از خزش در طی حادثه شدید یک رآکتور آبی‌تحت‌فشار به‌جهت انتقال مواد رادیواکتیو به محیط‌زیست اهمیت ویژه‌ای برخوردار است. دنبال شدید، حرارت واپاشی منتقل‌شده سایر قسمت‌های سیستم‌خنک‌کننده رآکتور می‌تواند منجر گرم‌شدن سازه‌های سیستم خنک‌کننده (RCS) و خرابی نواحی تحت‌فشار آسیب‌پذیر شود. قطع‌کامل‌برق (SBO) بدون اقدامات اپراتور (توالی TMLB) یکی محتمل‌ترین سناریوهایی است که ممکن یکپارچ...
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