a nested polymerase chain reaction (pcr) assay with two pairs of primers was applied for the detection of m. tuberculosis complex dna in specimens of 51 patients. of these, 31 clinically diagnosed patients (group i) recruited from july 1996 to june 1997 were given antituherculous chemotherapy from one to six months with standard daily regimen (isaniazid, 5 mg/kg; rifampin, 10 mg/kg; pyrazinamit...

Background and Aims: Canine distemper (CD) is a deadly infectious disease of Canidae family. CD is a multi-systemic viral disease and is specified by wide range of clinical symptoms. The manifestations are not always indicative of CD, therefore a laboratory confirmation is necessary for suspected cases. Materials and Methods: Different clinical specimens of 19 CD suspected unvaccinated dogs wer...

objective: polymerase chain reaction (pcr) is one of the most important progress in the field of molecular biology and diagnosis. despite simplicity in concept, the reaction needs complex interaction between target sequence, primers, dntps and dna polymerase for a successful amplification and diagnosis. for the detection of rna viruses highly sensetive and specific technique is required. hence ...

abstract life-threatening infections caused by the encapsulated fungal pathogen cryptococcus neoformans have been increasing steadily over the past 10 years. cryptococcus neoformans is recognized as the most frequent fungal infection of the central nervous system (cns) in immunocompetent as well as immunocompromised patients. we report the development of a semi-nested- pcr-based assay for the d...

background and purpose: fungal keratitis is a suppurative, ulcerative, and sight-threatening infection of the cornea that sometimes leads to blindness. the aims of this study were: recuperating facilities for laboratory diagnosis, determining the causative microorganisms, and comparing conventional laboratory diagnostic tools and semi-nested pcr. materials and methods: sampling was conducted in...

outbreaks causing mass mortalities have been observed in the golden grey mullet (liza auratus) population in southern coastal area of the caspian sea since 2002. the clinical signs include neurological abnormalities, such as erratic swimming behavior, spiral swimming, belly-up at rest and over inflation of the swim bladder. three hundred and twelve samples of moribund fish with sizes that range...