Tumour necrosis factor α (TNFα) is involved in the pathogenesis of prostate cancer, a disease where disturbances in the endocannabinoid system are seen. In the present study we have investigated whether treatment of DU145 human prostate cancer cells affects anandamide (AEA) catabolic pathways. Additionally, we have investigated whether cyclooxygenase-2 (COX-2) can regulate the uptake of AEA int...

Prostate cancer (PCa) is a leading cause for death in men and the most commonly diagnosed malignancy globally. MicroRNA (miR)‑583 expression levels have been discovered to be downregulated recurrent PCa samples compared with non‑recurrent cases. However, precise functions pathogenic mechanism of miR‑583 development are vague, thus aim present study was investigate these. The Janus kinase 1 (JAK...

the purpose of this study is to investigate the effects of quercetin as an inhibitor of heat shock proteins and hyperthermia on the induced dna damages and colony formation ability of du145 tumor spheroid culture. du145 cells were cultured as spheroids. on day11, spheroids with mean diameter 100 μm were treated with different concentration of quercetin for 24 hours and then exposed to hyperther...

Prostate cancer is one of the most common malignancies in men, and it urgently demands precise interventions that target the signaling pathways implicated in its initiation, progression, and metastasis. The Notch-1 signaling pathway is closely associated with the pathophysiology of prostate cancer. This study investigated the antitumor effects and mechanisms of curcumin, which is a well-known n...

The epidermal growth factor receptor (EGFR) network has rich targets for prostate cancer killing. Herein we evaluated the effects of combining the EGFR inhibition and radiation on DU145 prostate cancer. We treated DU145 prostate cancer cells with various doses of anti-EGFR antibody (C225) and gamma-irradiation (RAD). The effects of the treatment on cell viability and growth were assessed with c...

Cancer metastasis is the process of dissemination cancer cells from their original site and localization in distant sites. During this journey, metastatic have to undergo several phenotypical/ genotypical changes for acquiring cell migration, invasion, attachment properties etc. These steps can be partially/fully mimicked under vivo vitro conditions. Here, we injected prostate line, DU145, into...

The present study was undertaken to compare the relationship between response to exogenous epidermal growth factor (EGF) and the expression of the EGF-receptor (EGF-R) in an androgen sensitive (LNCaP) and insensitive (DU145) prostate cancer cell line. Although both cell lines demonstrated a single EGF-R binding site of similar high affinities (mean dissociation constant (Kd) ± S.D. for DU145 = ...

A benzopentasulfane was synthesized in 8 steps with a ceramide attached through an amide bond to the 7-position of the heterocycle structure. The anticancer activity of this synthetic ceramide-benzopolysulfane drug conjugate was analyzed against five human cancer cell lines MDA-MB-231 (breast), DU145 (prostate), MIA PaCa-2 (pancreas), HeLa (cervix), and U251 (glioblastoma). The ceramide-benzopo...

AIM To determine the mechanisms of glucocorticoids in inhibiting advanced prostate cancer growth. METHODS The cell proliferation and cell cycle of prostate cancer DU145 cells following dexamethasone treatment were determined by proliferation assay and fluorescence-activated cell sorter. Western blot analysis was carried out to evaluate the effects of dexamethasone on phosphorylation of extrac...

The aims of this study were to investigate the impact of soybean isoflavones (SIFs) on the cellular oxidative stress levels in hormone-sensitive type (LNCap) and hormone-insensitive type (DU145) of prostate cancer, and to elucidate the main components and their roles in the transformation of androgen dependency (AD)/ androgen independency (AI). The LNCap and DU145 cells were treated with differ...