Carbapenem resistance presents severe threat to the treatment of multidrug resistant Pseudomonas aeruginosa infections. The study was undertaken to investigate the role of efflux pumps in conferring meropenem resistance and effect of single dose exposure of meropenem on transcription level of mexA gene in clinical isolates of P. aeruginosa from a tertiary referral hospital of India. Further, in...

Multidrug efflux pumps contribute to multiple antibiotic resistance in Pseudomonas aeruginosa. Pump expression usually has been quantified by Western blotting. Quantitative real-time polymerase chain reaction has been developed to measure mRNA expression for genes of interest. Whether this method correlates with pump protein quantities is unclear. We devised a real-time PCR for mRNA expression ...

Pseudomonas aeruginosa, a Gram-negative, rod-shaped bacterium, holds prominent position as an antibiotic-resistant priority pathogen, according to the World Health Organization. Particularly prevalent in healthcare settings, this bacterium acts opportunistic causing nosocomial infections. The significant antibiotic resistance observed P. aeruginosa is multifactorial, encompassing intrinsic, acq...

According to the prevalence of multidrug resistance bacteria, especially Pseudomonas aeruginosa, in which essential mechanism drug is ability possess an efflux pump by extrusion antimicrobial agents usually occurs, this study aims detect presence mexB gene some local isolates bacteria that show towards three antibiotics, out five. Sensitivity test antibiotics was performed on all using meropene...