In skeletal muscle, an increased expression of insulin like growth factor-I isoforms IGF-IEa and mechano-growth factor (MGF) combined with downregulation of myostatin is thought to be essential for training-induced hypertrophy. However, the specific effects of different contraction types on regulation of these factors in muscle are still unclear, and in tendon the functions of myostatin, IGF-IE...

The size and shape of leaves are influenced by the progression of the cell cycle arrest front (AF). However, the AF progression with leaf growth has not been characterized quantitatively. Moreover, the mechanism linking AF progression and genetic factors is not fully understood. Recently, it was proposed that a KLUH/CYP78A5 (KLU)-dependent signal acts as a mobile growth factor (MGF) for cell pr...

In the present study, we analyzed the capacity of CD34+/CD36- sorted bone marrow cells of myelodysplasia patients (n = 4) to differentiate along the erythroid lineage in the presence of erythropoietin (Epo) and mast cell growth factor (MGF). Two subgroups could be identified. In 6 patients, a normal number of burst-forming units-erythroid (BFU-Es) were cultured from CD34+/CD36- sorted cells. Ce...

Pluripotent hematopoietic stem cells (PHSC) are very rare cells whose functional capabilities can only be analyzed indirectly. For a better understanding and possible manipulation of mechanisms that regulate self-renewal and commitment to differentiation of PHSC, it is necessary to purify these cells and to develop assays for their growth in vitro. In the present study, a rapid and simple, wide...

Thioglycolate-stimulated mouse peritoneal macrophages cultured in the presence of macrophage growth factor (MGF) will continue to proliferate when they are removed from culture dishes with the local anesthetic lidocaine and subcultured. The number of times the cells can be subcultured and remain in a proliferative state is dependent on the number of previous cell divisions. One precursor cell (...

Human mast cell growth factor (MGF) complementary DNAs (cDNAs) were cloned from HeLa cells using the polymerase chain reaction with oligonucleotides corresponding to murine and human MGF sequences. Sequencing of the cloned human MGF polymerase chain reaction products revealed two types of cDNA: a full length form corresponding in size to the murine cDNA, and an alternately spliced clone with a ...

Escherichia coli has dispensable genome regions and eliminating them may improve cell use by reducing unnecessary metabolic pathways and complex regulatory networks. Although several strains with reduced genomes have already been constructed, there have been no reports of strains constructed with deletions assayed for influence on growth. To retain robust growth and fundamental metabolic pathwa...

analysis showed that this was a splice variant of the insulin-like growth factor-I (IGF-I) gene, although its 3 exons were different from the liver or systemic type of IGF-I. However, it was noted that muscle also expresses the systemic type of IGF-I (IGF-IEa). The terminology for the IGF-I variants is a problem when attempting to apply it to nonhepatic tissues and to different species. Also, w...

analysis showed that this was a splice variant of the insulin-like growth factor-I (IGF-I) gene, although its 3 exons were different from the liver or systemic type of IGF-I. However, it was noted that muscle also expresses the systemic type of IGF-I (IGF-IEa). The terminology for the IGF-I variants is a problem when attempting to apply it to nonhepatic tissues and to different species. Also, w...